Iran University of Medical Sciences, Deputy of Health, Tehran, Iran.
Tabriz University of Medical Sciences, Infectious and Tropical Diseases Research Center, Tabriz, Iran.
Braz J Infect Dis. 2022 May-Jun;26(3):102354. doi: 10.1016/j.bjid.2022.102354. Epub 2022 Apr 25.
One of the hallmarks of COVID-19 is overwhelming inflammation, which plays a very important role in the pathogenesis of COVID-19. Thus, identification of inflammatory factors that interact with the SARS-CoV-2 can be very important to control and diagnose the severity of COVID-19. The aim of this study was to investigate the expression patterns of inflammation-related non-coding RNAs (ncRNAs) including MALAT-1, NEAT-1, THRIL, and miR-155-5p from the acute phase to the recovery phase of COVID-19.
Total RNA was extracted from Peripheral Blood Mononuclear Cell (PBMC) samples of 20 patients with acute COVID-19 infection and 20 healthy individuals and the expression levels of MALAT-1, NEAT-1, THRIL, and miR-155-5p were evaluated by real-time PCR assay. Besides, in order to monitor the expression pattern of selected ncRNAs from the acute phase to the recovery phase of COVID-19 disease, the levels of ncRNAs were re-measured 6‒7 weeks after the acute phase.
The mean expression levels of MALAT-1, THRIL, and miR-155-5p were significantly increased in the acute phase of COVID-19 compared with a healthy control group. In addition, the expression levels of MALAT-1 and THRIL in the post-acute phase of COVID-19 were significantly lower than in the acute phase of COVID-19. According to the ROC curve analysis, these ncRNAs could be considered useful biomarkers for COVID-19 diagnosis and for discriminating between acute and post-acute phase of COVID-19.
Inflammation-related ncRNAs (MALAT-1, THRIL, and miR-150-5p) can act as hopeful biomarkers for the monitoring and diagnosis of COVID-19 disease.
COVID-19 的一个特征是炎症的压倒性反应，这在 COVID-19 的发病机制中起着非常重要的作用。因此，鉴定与 SARS-CoV-2 相互作用的炎症因子对于控制和诊断 COVID-19 的严重程度非常重要。本研究旨在从急性到恢复期 COVID-19 调查炎症相关非编码 RNA（ncRNA）包括 MALAT-1、NEAT-1、THRIL 和 miR-155-5p 的表达模式。
从 20 名急性 COVID-19 感染患者和 20 名健康个体的外周血单核细胞（PBMC）样本中提取总 RNA，并通过实时 PCR 检测 MALAT-1、NEAT-1、THRIL 和 miR-155-5p 的表达水平。此外，为了监测 COVID-19 疾病急性到恢复期选定 ncRNA 的表达模式，在急性后 6-7 周重新测量 ncRNA 的水平。
与健康对照组相比，COVID-19 急性期中 MALAT-1、THRIL 和 miR-155-5p 的平均表达水平显着升高。此外，COVID-19 急性后期中 MALAT-1 和 THRIL 的表达水平显着低于急性期中的表达水平。根据 ROC 曲线分析，这些 ncRNA 可被视为 COVID-19 诊断和区分急性和急性后 COVID-19 的有用生物标志物。
炎症相关 ncRNA（MALAT-1、THRIL 和 miR-155-5p）可作为监测和诊断 COVID-19 疾病的有希望的生物标志物。
