College of Food Science, Shenyang Agricultural University, Shenyang 110866, PR China.
College of Food Science, Shenyang Agricultural University, Shenyang 110866, PR China.
Int J Biol Macromol. 2023 Dec 1;252:126321. doi: 10.1016/j.ijbiomac.2023.126321. Epub 2023 Aug 14.
The purpose of this study is to identify and characterize mirnas in mammalian exosomes. Using Illumina sequencing technology, we sequenced miRNAs in the exosomes of mammalian human milk, bovine milk, and donkey milk. 36 known mature miRNAs and 256 novel miRNAs were identified in human milk. 61 known mature miRNAs and 346 novel miRNAs were identified in milk. 16 known mature miRNAs and 196 novel miRNAs were identified in donkey milk, and miRNAs target genes were predicted. Gene Ontology analysis showed that the miRNAs of human, bovine and donkey milk exosomes all labeled the functions related to body metabolism. Kyoto Encyclopedia pathway analysis showed that human, bovine and donkey milk miRNAs enriched AGE-RAGE signaling pathway in Complications of diabetes. Diabetes is a Metabolic disorder. Based on this pathway, we screened out hsa-miR-8485, bta-miR-342, miR-29c and other genes related to diabetes. This study has a new understanding of the physiological function of mammalian milk miRNAs, and also provides a new way to explore diabetes related miRNAs.
本研究旨在鉴定和描述哺乳动物外泌体中的 mirnas。我们使用 Illumina 测序技术，对人乳、牛乳和驴乳的外泌体中的 miRNAs 进行了测序。在人乳中鉴定出 36 个已知成熟的 miRNAs 和 256 个新的 miRNAs。在乳中鉴定出 61 个已知成熟的 miRNAs 和 346 个新的 miRNAs。在驴乳中鉴定出 16 个已知成熟的 miRNAs 和 196 个新的 miRNAs，并预测了 miRNAs 的靶基因。GO 分析表明，人乳、牛乳和驴乳外泌体的 miRNAs 均标记与机体代谢相关的功能。京都基因与基因组百科全书通路分析表明，人乳、牛乳和驴乳的 miRNAs 富集了糖尿病并发症中的 AGE-RAGE 信号通路。糖尿病是一种代谢紊乱。基于该通路，我们筛选出与糖尿病相关的 hsa-miR-8485、bta-miR-342、miR-29c 等基因。本研究对哺乳动物乳 miRNAs 的生理功能有了新的认识，也为探索糖尿病相关 miRNAs 提供了新的途径。
