Szerdahelyi P, Kása P
Histochemistry. 1986;85(4):349-52. doi: 10.1007/BF00493488.
Earlier, widely used histochemical methods for the demonstration of copper are capable of detecting only extremely high tissue levels of this metal (generally only in pathological states, e.g. Wilson's disease, or in cases of copper intoxication), because of their low sensitivity. The specificity of these methods has also proved to be unsatisfactory. We present a new method based on the release of bound (unreactive) copper by trichloroacetic acid, its primary precipitation using magnesium dithizonate, and intensification of the staining (secondary precipitation) using silver nitrate. Using this method, copper is demonstrable in various tissues of normal rats (brain, stomach, liver, small intestine, spleen, pancreas, kidneys) in the form of reddish to pink staining. This method can also be applied to locate pathologically high levels of copper.
早期，广泛使用的用于显示铜的组织化学方法，由于其灵敏度低，只能检测到这种金属在组织中的极高含量（通常仅在病理状态下，如威尔逊氏病或铜中毒病例中）。这些方法的特异性也已证明不尽人意。我们提出了一种新方法，该方法基于用三氯乙酸释放结合的（无反应性的）铜，用镁双硫腙进行初次沉淀，并使用硝酸银增强染色（二次沉淀）。使用这种方法，可在正常大鼠的各种组织（脑、胃、肝、小肠、脾、胰腺、肾）中以红至粉红色染色的形式显示铜。该方法也可用于定位病理性高水平的铜。
