Li Song, Han Dexing, Chen Xiaowei, Zheng Dexiang, Cai Yimei, Lin Dongling, Zhang Xuan, Ke Peifeng, Qu Pinghua, Chen Cha
The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China.
Department of Clinical Laboratory, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, China.
Heliyon. 2023 Aug 7;9(8):e18990. doi: 10.1016/j.heliyon.2023.e18990. eCollection 2023 Aug.
The matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been widely applied in routine clinical microbiology laboratories as an efficient and reliable technique for diagnostic purpose. In this work, we evaluated the performance of the newly developed Zybio EXS3000 (Zybio Inc., China) in microbial identification and compared it with VITEK MS (bioMérieux, France). For this study, a total of 1340 isolates from various clinical specimens were collected. These isolates were analyzed simultaneously on both EXS3000 and VITEK MS. The inconsistent or unidentifiable data were further identified using the help of either 16S rRNA gene or ITS region sequencing. During the study, we observed that EXS3000 and VITEK MS provided positive confirmatory diagnostics for 95.0% and 96.5% of the isolates, respectively, which were consistent with the sequencing results. However, it is worth noting that the EXS3000 system needs to improve the identification performance of in the follow-up. There are no significant differences between the two devices in terms of microbial identification performance. The advantage of EXS3000 over VITEK MS is in its ability to perform in significantly lesser time period. In conclusion, the results of this investigation showed that EXS3000 can be used to identify microorganisms in clinical microbiology laboratories.
基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）作为一种高效可靠的诊断技术已在临床微生物学常规实验室中广泛应用。在本研究中，我们评估了新开发的Zybio EXS3000（中国Zybio公司）在微生物鉴定方面的性能，并将其与VITEK MS（法国生物梅里埃公司）进行比较。本研究共收集了1340株来自各种临床标本的分离株。这些分离株同时在EXS3000和VITEK MS上进行分析。对于不一致或无法鉴定的数据，借助16S rRNA基因或ITS区域测序进一步鉴定。在研究过程中，我们观察到EXS3000和VITEK MS分别对95.0%和96.5%的分离株提供了阳性确诊诊断，这与测序结果一致。然而，值得注意的是，EXS3000系统在后续需要提高对[此处原文缺失具体内容]的鉴定性能。在微生物鉴定性能方面，这两种设备之间没有显著差异。EXS3000相对于VITEK MS的优势在于其能够在显著更短的时间内完成检测。总之，本调查结果表明EXS3000可用于临床微生物学实验室的微生物鉴定。
