石竹科A. Rich根对感染小鼠的抗疟作用。
Antimalarial Effect of the Root of A. Rich (Caryophyllaceae) on --Infected Mice.
作者信息
Atsbha Gebru Hagos, Balasubramanian Rajkapoor, Gebre Abadi Kahsu
机构信息
Department of Pharmacy, College of Health Sciences, Adigrat University, Adigrat, Ethiopia.
School of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, Ethiopia.
出版信息
Evid Based Complement Alternat Med. 2021 Mar 16;2021:8833865. doi: 10.1155/2021/8833865. eCollection 2021.
BACKGROUND
Malaria remains a major public health problem globally. Poor access to antimalarial drugs compounded with rapidly evolving drug resistance encourages researchers to continuously look for new drugs. Of importance, traditionally used medicines of plant origin are the highest priority as the ethnobotanical claim can be used as an important clue for its safety and efficacy profiles. A. Rich (Caryophyllaceae) has been traditionally used for malaria treatment in Ethiopia. Therefore, this study was aimed to evaluate the antimalarial activity of the plant against --infected (ANKA strain) Swiss albino mice.
METHODS
The dried powdered root of was extracted using 80% methanol. The crude extract was fractionated using chloroform, ethyl acetate, and distilled water that have different affinities to plant phytoconstituents. The antimalarial activities of the crude extract were evaluated using 4-day suppressive, prophylactic, and curative tests. The antimalarial activity of the solvent fractions was evaluated in a 4-day suppressive test. The oral acute toxicity of the crude extract was also determined according to the OECD guidelines.
RESULTS
The percentage of parasite suppression on the crude extract was 31.02%, 35.82%, and 39.23% in prophylactic, curative, and 4-day suppressive tests, respectively, at the tested dose level of 400 mg/kg. The percentages of chemosuppression of the solvent fractions (400 mg/kg) were 43.07%, 42.61%, and 38.38% in aqueous, ethyl acetate, and chloroform fractions, respectively. Both the crude extract and solvent fractions also significantly prolonged survival time except in the prophylactic test. In addition, prevention of weight loss and reduction in temperature and packed cell volume (PCV) were observed in crude extract as well as solvent fractions. The acute toxicity test of the plant extract also exhibited no sign of toxicity.
CONCLUSION
The result indicated that has a significant antimalarial activity, justifying the traditional use of the plant material for treatment of malaria.
背景
疟疾仍是全球主要的公共卫生问题。抗疟药物获取困难，再加上耐药性迅速演变，促使研究人员不断寻找新药。重要的是，传统使用的植物源药物是重中之重，因为民族植物学主张可作为其安全性和有效性概况的重要线索。在埃塞俄比亚，A. Rich（石竹科）传统上用于治疗疟疾。因此，本研究旨在评估该植物对感染（ANKA株）瑞士白化小鼠的抗疟活性。
方法
使用80%甲醇提取干燥的A. Rich粉末状根。粗提物用对植物植物成分具有不同亲和力的氯仿、乙酸乙酯和蒸馏水进行分级分离。粗提物的抗疟活性通过4天抑制、预防和治疗试验进行评估。溶剂级分的抗疟活性在4天抑制试验中进行评估。粗提物的口服急性毒性也根据经合组织指南进行测定。
结果
在400mg/kg的测试剂量水平下，粗提物在预防、治疗和4天抑制试验中的寄生虫抑制率分别为31.02%、35.82%和39.23%。溶剂级分（400mg/kg）的化学抑制率在水相、乙酸乙酯和氯仿级分中分别为43.07%、42.61%和38.38%。除预防试验外，粗提物和溶剂级分均显著延长了存活时间。此外，在粗提物和溶剂级分中均观察到预防体重减轻以及体温和红细胞压积（PCV）降低。植物提取物的急性毒性试验也未显示毒性迹象。
结论
结果表明A. Rich具有显著的抗疟活性，证明了该植物材料用于治疗疟疾的传统用途。
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