Detection of NDM-1 and OXA-10 Co-Producing Clinical Isolate.

BACKGROUND

The coexistence of with other resistance determinants is rarely reported for . Therefore, this study investigates the phenotypic and genetic characteristics of a multidrug-resistant strain YQ150713.

METHODS

YQ150713 was identified as carrying . S1-pulsed-field gel electrophoresis (S1-PFGE), Southern blotting, and conjugation experiments were used to determine plasmid characteristics. An antimicrobial susceptibility test was conducted. The complete genomic sequence of YQ150713 was obtained using Illumina NovaSeq 6000 and Oxford nanopore platforms. To further characterize the phylogenetic structure of YQ150713, average nucleotide identity (ANI) and phylogenetic analyses were conducted.

RESULTS

The S1-PFGE, Southern blot, and conjugation assays have confirmed that the isolate YQ150713 contains the gene on a conjugative plasmid pYQ150713-NDM-1. Antimicrobial susceptibility testing has indicated that strain YQ150713 was resistant to various common antibiotics, except aztreonam and fosfomycin. Bioinformatics analysis has further shown that pYQ150713-NDM-1 was a novel plasmid with a size of 265,883 bp, and and were co-located on it. Phylogenetic analysis suggesting has spread widely throughout the world.

CONCLUSION

In this study, and were co-localized on a novel plasmid pYQ150713-NDM-1 with a horizontal transfer function. To reduce the risk of the dissemination of such isolates in clinical settings, more surveillance will be required in the future.