Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku, Tokyo 162-8655, Japan.
BMC Infect Dis. 2014 Feb 3;14:56. doi: 10.1186/1471-2334-14-56.
Drug-resistant Providencia rettgeri producing metallo-β-lactamase and 16S rRNA methylase has been reported in several countries. We analyzed P. rettgeri clinical isolates with resistance to carbapenems and aminoglycosides in a hospital in Nepal.
Five clinical isolates of multidrug-resistant P. rettgeri were obtained in a hospital in Nepal. Antimicrobial susceptibilities were determined using the microdilution method and entire genomes were sequenced to determine drug-resistant genes. Epidemiological analysis was performed by pulsed-field gel electrophoresis.
Four of the 5 isolates were resistant to carbapenems (imipenem and meropenem), with MICs ≥16 mg/L, with the remaining isolate showing intermediate resistance to imipenem, with an MIC of 2 mg/L and susceptibility to meropenem with an MIC ≤1 mg/L. All 5 isolates had blaVEB-1. Of the 4 carbapenem-resistant strains, 3 had blaNDM-1 and 1 had blaOXA-72. All isolates were highly resistant to aminoglycosides (MICs ≥1,024 mg/L) and harbored armA. As the result of pulsed-field gel electrophoresis pattern analysis in the 5 P. rettgeri isolates, 4 had identical PFGE patterns and the fifth showed 95.7% similarity.
This is the first report describing multidrug-resistant P. rettgeri strains harboring blaNDM-1 or blaOXA-72 and armA isolated from patients in Nepal.
产金属β-内酰胺酶和 16S rRNA 甲基酶的耐药福氏普罗威登斯菌已在多个国家报道。我们分析了尼泊尔一家医院对碳青霉烯类和氨基糖苷类药物耐药的普罗威登斯菌临床分离株。
从尼泊尔一家医院获得 5 株多药耐药福氏普罗威登斯菌临床分离株。采用微量稀释法测定抗菌药物敏感性，对全基因组进行测序以确定耐药基因。采用脉冲场凝胶电泳进行流行病学分析。
5 株分离株中有 4 株对碳青霉烯类（亚胺培南和美罗培南）耐药，MICs≥16mg/L，其余 1 株对亚胺培南中介耐药，MIC 为 2mg/L，对美罗培南敏感，MIC≤1mg/L。5 株分离株均携带 blaVEB-1。4 株碳青霉烯类耐药株中有 3 株携带 blaNDM-1，1 株携带 blaOXA-72。所有分离株均对氨基糖苷类高度耐药（MICs≥1024mg/L），并携带 armA。5 株福氏普罗威登斯菌的脉冲场凝胶电泳图谱分析结果显示，4 株具有相同的 PFGE 模式，第 5 株具有 95.7%的相似性。
这是首次报道从尼泊尔患者中分离出携带 blaNDM-1 或 blaOXA-72 和 armA 的多药耐药福氏普罗威登斯菌。
