Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, 450001, Henan, China.
State Key Laboratory of Cotton Biology, Cotton Research Institute of Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China.
BMC Genomics. 2023 Aug 22;24(1):474. doi: 10.1186/s12864-023-09524-w.
The glyoxalase system includes glyoxalase I (GLXI), glyoxalase II (GLXII) and glyoxalase III (GLXIII), which are responsible for methylglyoxal (MG) detoxification and involved in abiotic stress responses such as drought, salinity and heavy metal.
In this study, a total of 620 GLX family genes were identified from 21 different plant species. The results of evolutionary analysis showed that GLX genes exist in all species from lower plants to higher plants, inferring that GLX genes might be important for plants, and GLXI and GLXII account for the majority. In addition, motif showed an expanding trend in the process of evolution. The analysis of cis-acting elements in 21 different plant species showed that the promoter region of the GLX genes were rich in phytohormones and biotic and abiotic stress-related elements, indicating that GLX genes can participate in a variety of life processes. In cotton, GLXs could be divided into two groups and most GLXIs distributed in group I, GLXIIs and GLXIIIs mainly belonged to group II, indicating that there are more similarities between GLXII and GLXIII in cotton evolution. The transcriptome data analysis and quantitative real-time PCR analysis (qRT-PCR) show that some members of GLX family would respond to high temperature treatment in G.hirsutum. The protein interaction network of GLXs in G.hirsutum implied that most members can participate in various life processes through protein interactions.
The results elucidated the evolutionary history of GLX family genes in plants and lay the foundation for their functions analysis in cotton.
糖氧还蛋白系统包括糖氧还酶 I（GLXI）、糖氧还酶 II（GLXII）和糖氧还酶 III（GLXIII），它们负责甲基乙二醛（MG）解毒，并参与非生物胁迫反应，如干旱、盐度和重金属。
在这项研究中，从 21 种不同的植物中鉴定出了总共 620 个 GLX 家族基因。进化分析的结果表明，GLX 基因存在于从低等植物到高等植物的所有物种中，这表明 GLX 基因可能对植物很重要，并且 GLXI 和 GLXII 占大多数。此外，基序在进化过程中表现出扩张的趋势。对 21 种不同植物的顺式作用元件分析表明，GLX 基因的启动子区域富含植物激素和生物及非生物胁迫相关元件，表明 GLX 基因可以参与多种生命过程。在棉花中，GLXs 可以分为两组，大多数 GLXI 分布在第一组，GLXII 和 GLXIII 主要属于第二组，这表明在棉花进化过程中 GLXII 和 GLXIII 之间有更多的相似性。转录组数据分析和定量实时 PCR 分析（qRT-PCR）表明，GLX 家族的一些成员会对 G. hirsutum 中的高温处理产生反应。G. hirsutum 中 GLXs 的蛋白质相互作用网络表明，大多数成员可以通过蛋白质相互作用参与各种生命过程。
该结果阐明了植物中 GLX 家族基因的进化历史，为其在棉花中的功能分析奠定了基础。
