Cloning and Functional Analysis of Glyoxalase I Gene in L.

Glyoxalase I (GLYI) is a key enzyme that detoxifies methylglyoxal, a toxic byproduct of glycolysis, and is essential for plant pollination. However, the genome-wide identification and functional analysis of in L. () remain limited. This study identified 17 genes (-) from the genome. The self-compatible line 039-1 and the self-incompatible line GAU-28-5 were used as experimental materials, and Real-Time Quantitative Reverse Transcription PCR (RT-qPCR) was performed to examine the effect of genes on self-compatibility in winter . Preliminary results showed that exhibited significant tissue specificity, with higher expression in the flowers of 039-1 compared to GAU-28-5. The open reading frame of (852 bp) was cloned from both 039-1 and GAU-28-5 cDNA, with no base mutations observed between the two lines. RT-qPCR revealed higher expression in the stigma of 039-1 compared to GAU-28-5. Based on the functional conservation and sequence homology, is speculated to play a similar role to that of in methylglyoxal detoxification and stress response. Furthermore, the knockout of resulted in reduced silique lengths and seed numbers. These findings suggest that is involved in the self-compatibility response in and promotes the silique length and seed number in the Arabidopsis mutant, providing a basis for further research on the mechanisms of self-compatibility in .