评价 Autof ms1000 质谱仪在丝状真菌快速临床鉴定中的应用。
Evaluation of the Autof ms1000 mass spectrometry for rapid clinical identification of filamentous fungi.
机构信息
Department of Laboratory Medicine, West China Hospital, Sichuan University, No. 37 Guoxue Lane, Chengdu, 610041, China.
出版信息
BMC Microbiol. 2023 Aug 22;23(1):228. doi: 10.1186/s12866-023-02968-w.
BACKGROUND
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has revolutionized microbial identification. However, there is a lack of data on its performance in identifying filamentous fungi. The objective of our study was to evaluate the accuracy of the Autof ms1000 mass spectrometry for identifying filamentous fungi in the clinical microbiology laboratory.
RESULTS
Among 106 samples tested using the Autof ms1000 system, 101 (95.28%) were identified at the genus or species level, and 81 (76.41%) were accurately identified at the species level. Additionally, we developed a new rapid formic acid extraction method with simple pretreatment for filamentous fungi that saved time and provided accurate results.
CONCLUSIONS
The Autof ms1000 mass spectrometer proved to be a valuable tool for identifying filamentous fungi. However, upgrading the database is recommended for correctly identifying rare strains.
背景
基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）技术革新了微生物鉴定。然而，关于其在丝状真菌鉴定中的性能的数据还很缺乏。我们的研究目的是评估 Autof ms1000 质谱仪在临床微生物学实验室中鉴定丝状真菌的准确性。
结果
使用 Autof ms1000 系统检测的 106 个样本中，有 101 个（95.28%）在属或种水平上得到鉴定，81 个（76.41%）在种水平上得到准确鉴定。此外，我们开发了一种新的快速甲酸提取方法，对丝状真菌进行简单的预处理，节省了时间并提供了准确的结果。
结论
Autof ms1000 质谱仪被证明是一种鉴定丝状真菌的有价值的工具。然而，建议升级数据库以正确鉴定罕见菌株。
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