Department of Laboratory Medicine, Seoul National University College of Medicine, 03080 Seoul, Republic of Korea.
Department of Laboratory Medicine, Seoul National University Hospital, 03080 Seoul, Republic of Korea.
Biomed Res Int. 2021 Mar 3;2021:6667623. doi: 10.1155/2021/6667623. eCollection 2021.
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is widely used in clinical microbiology laboratories because it is cost-effective, reliable, and fast. This study is aimed at comparing the identification performance of the recently developed Autof ms1000 (Autobio, China) with that of the Bruker Biotyper (Bruker Daltonics, Germany). From January to June 2020, 205 preserved strains and 302 clinical isolates were used for comparison. Bacteria were tested with duplicates of the direct transfer method, and formic acid extraction was performed if the results were not at the species level. Fungi were tested with formic acid extraction followed by ethanol extraction methods. 16S rRNA or ITS region sequence analysis was performed on isolates that could not be identified by any of the instruments and on isolates that showed inconsistent results. The time to result of each instrument was also compared. Among preserved strains, species-level identification results were obtained in 202 (98.5%) strains by the Autof ms1000 and 200 (97.6%) strains by the Bruker Biotyper. Correct identification at the species/complex level was obtained for 200 (97.6%) strains by the Autof ms1000 and for 199 (97.1%) strains by the Bruker Biotyper. Among clinical isolates, species-level identification results were obtained in 301 (99.7%) strains and 300 (99.3%) strains by the Autof ms1000 and Bruker Biotyper, respectively. Correct identification at the species/complex level was achieved for 299 (99.0%) strains by the Autof ms1000 and for 300 (99.3%) strains by the Bruker Biotyper. The time to analyze 96 spots was approximately 14 min for the Autof ms1000 and approximately 27 min for the Bruker Biotyper. The two instruments showed comparable performance for the routine identification of clinical microorganisms. In addition, the Autof ms1000 has a short test time, making it convenient for use in clinical microbiology laboratories.
基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）在临床微生物学实验室中得到广泛应用，因为它具有成本效益、可靠且快速的特点。本研究旨在比较最近开发的 Autof ms1000（Autobio，中国）与 Bruker Biotyper（Bruker Daltonics，德国）的鉴定性能。2020 年 1 月至 6 月，使用 205 株保存菌株和 302 株临床分离株进行比较。采用直接转移法对细菌进行两次重复检测，如果结果未达到种水平，则进行甲酸提取。采用甲酸提取后乙醇提取方法检测真菌。对任何仪器都无法鉴定的分离株以及结果不一致的分离株进行 16S rRNA 或 ITS 区序列分析。还比较了两种仪器的结果时间。在保存菌株中，Autof ms1000 获得 202 株（98.5%）菌株的种水平鉴定结果，Bruker Biotyper 获得 200 株（97.6%）菌株的种水平鉴定结果。Autof ms1000 获得 200 株（97.6%）菌株和 Bruker Biotyper 获得 199 株（97.1%）菌株的种/复合体水平的正确鉴定结果。在临床分离株中，Autof ms1000 获得 301 株（99.7%）菌株和 Bruker Biotyper 获得 300 株（99.3%）菌株的种水平鉴定结果。Autof ms1000 获得 299 株（99.0%）菌株和 Bruker Biotyper 获得 300 株（99.3%）菌株的种/复合体水平的正确鉴定结果。Autof ms1000 分析 96 个斑点的时间约为 14 分钟，Bruker Biotyper 约为 27 分钟。这两种仪器在常规鉴定临床微生物方面表现相当。此外，Autof ms1000 的测试时间较短，方便在临床微生物学实验室使用。
