Abdelhamid Gehad A, Abdel-Aal Amany A, Badawi Manal, Fahmy Mennat-Elrahman A, Abd-Alghany Asmaa R
Department of Medical Parasitology, Faculty of Medicine, Cairo University, Cairo, Egypt.
Department of Postgraduate Studies and Scientific Research, Armed Forces College of Medicine (AFCM), Cairo, Egypt.
J Parasit Dis. 2025 Jun;49(2):419-425. doi: 10.1007/s12639-024-01763-2. Epub 2024 Dec 6.
Recently, testing existing drugs for new therapeutic purposes has been highly recommended. Trichinellosis is a parasitic infection caused by roundworms from the genus . Treating trichinellosis is facing many challenges and offering new pharmacological therapeutics is needed. In this study, Amiodarone, the antiarrhythmic medication was repurposed for the first time for treating the muscle phase of trichinellosis in experimental mice. Thirty-five mice were used and divided as follows; normal, infected, infected, and treated with Albendazole (ALB), and the infected and treated with Amiodarone (AMD). Thirty-five days post inoculation of infection and after euthanasia; the diaphragms of all mice were subjected to histopathological examination while the whole muscle masses of the infected and infected-treated mice were subjected to digestion and examination for assessing the number of larvae per gram. Compared to ALB, AMD showed the best results in this study. A significant reduction of muscle larval burden (71.43%, versus ALB 62.36%; <.001) and improvement of the diaphragmatic histopathological changes with a significant reduction of inflammatory infiltrates ( <.001). According to our results, AMD showed an anti-inflammatory plus antiparasitic action against the muscular phase of experimental trichinellosis. In addition, ALB, the drug of choice till now should be tested in combination with other treatments to improve its intestinal absorption and subsequently its anti-larval efficacy, plus reducing its consequential severe inflammatory reaction.
最近，强烈建议对现有药物进行新的治疗用途测试。旋毛虫病是由旋毛虫属的蛔虫引起的寄生虫感染。治疗旋毛虫病面临许多挑战，因此需要提供新的药物疗法。在本研究中，抗心律失常药物胺碘酮首次被重新用于治疗实验小鼠旋毛虫病的肌肉期。使用了35只小鼠并分为以下几组：正常组、感染组、感染并用阿苯达唑（ALB）治疗组以及感染并用胺碘酮（AMD）治疗组。感染接种35天后并实施安乐死后；对所有小鼠的膈肌进行组织病理学检查，同时对感染组和感染治疗组小鼠的整个肌肉团进行消化和检查，以评估每克肌肉中的幼虫数量。与阿苯达唑相比，胺碘酮在本研究中显示出最佳效果。肌肉幼虫负荷显著降低（71.43%，而阿苯达唑为62.36%；P<0.001），膈肌组织病理学变化得到改善，炎症浸润显著减少（P<0.001）。根据我们的结果，胺碘酮对实验性旋毛虫病的肌肉期显示出抗炎和抗寄生虫作用。此外，至今的首选药物阿苯达唑应与其他治疗方法联合进行测试，以改善其肠道吸收，进而提高其抗幼虫疗效，并减少其随之而来的严重炎症反应。
