Silber S J, Miller J F, Morris P J
Transplantation. 1976 Aug;22(2):160-6. doi: 10.1097/00007890-197608000-00011.
Selective recruitment of antigen-sensitive cells (ASC) into the spleen as a method of inducing specific suppression was attempted by intravenous injection of either DA or Lewis spleen cells 24 hr before a (DA X Lewis)F1 renal allograft into a Lewis or DA recipient, either with or without a splenectomy. This led to suppression of rejection in the DA recipient and delayed rejection in the Lewis recipient. Splenectomy produced a minimal augmentation effect. Assay of graft-versus-host (GVH) reactions in (DA X Lewis)F1 rats by a popliteal node assay showed that injection of allogeneic DA or Lewis spleen cells 48 hr before the assay significantly reduced the reaction produced by node lymphocytes but not spleen lymphocytes, suggesting a loss of ASC from the lymph nodes. Lewis spleen allografts did not produce such a significant reduction in the GVH reactivity of DA node lymphocytes as intravenous Lewis cells, whereas DA spleen allografts led to an increased GVH reactivity of Lewis node lymphocytes. From these studies, it is not possible to attribute the suppression produced by the intravenous injection of allogeneic cells to selective recruitment of antigen-sensitive cells to the spleen.
在将（DA×Lewis）F1肾移植给Lewis或DA受体之前24小时，通过静脉注射DA或Lewis脾细胞，尝试将抗原敏感细胞（ASC）选择性募集到脾脏中，以此作为诱导特异性抑制的一种方法，受体有的进行了脾切除术，有的没有。这导致DA受体中的排斥反应受到抑制，Lewis受体中的排斥反应延迟。脾切除术产生的增强作用最小。通过腘淋巴结试验对（DA×Lewis）F1大鼠的移植物抗宿主（GVH）反应进行检测，结果显示在试验前48小时注射同种异体DA或Lewis脾细胞可显著降低淋巴结淋巴细胞产生的反应，但对脾淋巴细胞产生的反应无此作用，这表明淋巴结中ASC有所减少。Lewis脾同种异体移植对DA淋巴结淋巴细胞的GVH反应性的降低程度不如静脉注射Lewis细胞，而DA脾同种异体移植则导致Lewis淋巴结淋巴细胞的GVH反应性增加。从这些研究中，无法将静脉注射同种异体细胞所产生的抑制作用归因于抗原敏感细胞向脾脏的选择性募集。
