Zhou Yu, Ruan Zuoxi, Fang Chong, Chen Xiaoyan, Xu Huijuan, Wang Zhongming, Yuan Zhenhong
Institute of Marine Biology, Shantou University, Shantou 510632, Guangdong, China.
Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, CAS Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, Guangdong, China.
Sheng Wu Gong Cheng Xue Bao. 2023 Aug 25;39(8):3125-3142. doi: 10.13345/j.cjb.220738.
C1 gases including CO, CO and CH, are mainly derived from terrestrial biological activities, industrial waste gas and gasification syngas. Particularly, CO and CH are two of the most important greenhouse gases contributing to climate change. Bioconversion of C1 gases is not only a promising solution to addressing the problem of waste gases emission, but also a novel route to produce fuels or chemicals. In the past few years, C1-gas-utilizing microorganisms have drawn much attention and a variety of gene-editing technologies have been applied to improve their product yields or to expand product portfolios. This article reviewed the biological characteristics, aerobic or anaerobic metabolic pathways as well as the metabolic products of methanotrophs, autotrophic acetogens, and carboxydotrophic bacteria. In addition, gene-editing technologies (e.g. gene interruption technology using homologous recombination, group Ⅱ intron ClosTron technology, CRISPR/Cas gene editing and phage recombinase-mediated efficient integration of large DNA fragments) and their application in these C1-gas-utilizing microorganisms were also summarized.
包括一氧化碳（CO）、二氧化碳（CO₂）和甲烷（CH₄）在内的C1气体主要来源于陆地生物活动、工业废气和气化合成气。特别是，CO₂和CH₄是导致气候变化的两种最重要的温室气体。C1气体的生物转化不仅是解决废气排放问题的一个有前景的解决方案，也是生产燃料或化学品的一条新途径。在过去几年中，利用C1气体的微生物受到了广泛关注，并且已经应用了多种基因编辑技术来提高它们的产物产量或扩大产物种类。本文综述了甲烷氧化菌、自养产乙酸菌和一氧化碳营养细菌的生物学特性、好氧或厌氧代谢途径以及代谢产物。此外，还总结了基因编辑技术（例如使用同源重组的基因中断技术、Ⅱ类内含子ClosTron技术、CRISPR/Cas基因编辑和噬菌体重组酶介导的大DNA片段高效整合）及其在这些利用C1气体的微生物中的应用。
