Bauer Franziska, Wolfschlaeger Immanuel, Geist Juergen, Fastner Jutta, Schmalz Carina Wiena, Raeder Uta
Aquatic Systems Biology Unit, Limnological Research Station Iffeldorf, Technical University of Munich, Hofmark 1-3, 82393 Iffeldorf, Germany.
German Environment Agency, Schichauweg 58, 12307 Berlin, Germany.
Toxics. 2023 Jul 25;11(8):643. doi: 10.3390/toxics11080643.
Cyanobacteria are favored by climate change and global warming; however, to date, most research and monitoring programs have focused on planktic cyanobacteria. Benthic cyanobacteria blooms also increase and pose a risk to animal and human health; however, there is limited knowledge of their occurrence, distribution and the toxins involved, especially in relation to their planktic conspecifics. Therefore, we analyzed the benthic and planktic life forms of cyanobacterial communities in 34 lakes in Germany, including a monitoring of cyanotoxins. Community analyses were based on microscopic examination and Illumina sequencing of the 16S rRNA gene. The analyses of cyanotoxins were carried out using LC-MS/MS and ELISA. Observed benthic mats containing cyanobacteria consisted mainly of and , being present in 35% of the lakes. Anatoxin was the most abundant cyanotoxin in the benthic samples, reaching maximum concentrations of 45,000 µg/L, whereas microcystin was the predominate cyanotoxin in the open-water samples, reaching concentrations of up to 18,000 µg/L. Based on the results, specific lakes at risk of toxic cyanobacteria could be identified. Our findings suggest that monitoring of benthic cyanobacteria and their toxins should receive greater attention, ideally complementing existing open-water sampling programs with little additional effort.
蓝藻受气候变化和全球变暖的影响；然而，迄今为止，大多数研究和监测项目都集中在浮游蓝藻上。底栖蓝藻水华也在增加，并对动物和人类健康构成风险；然而，人们对它们的发生、分布以及所涉及的毒素了解有限，尤其是与它们的浮游同类相比。因此，我们分析了德国34个湖泊中蓝藻群落的底栖和浮游生命形式，包括对蓝藻毒素的监测。群落分析基于显微镜检查和16S rRNA基因的Illumina测序。蓝藻毒素分析采用LC-MS/MS和ELISA进行。观察到的含有蓝藻的底栖藻席主要由[具体名称1]和[具体名称2]组成，存在于35%的湖泊中。鹅膏覃碱是底栖样本中含量最高的蓝藻毒素，最高浓度达到45000μg/L，而微囊藻毒素是敞水区样本中的主要蓝藻毒素，浓度高达18000μg/L。根据结果，可以确定存在有毒蓝藻风险的特定湖泊。我们的研究结果表明，对底栖蓝藻及其毒素的监测应得到更多关注，理想情况下只需付出很少的额外努力就能补充现有的敞水区采样项目。
