Sousa Patrícia, Lopes Bruna, Sousa Ana Catarina, Moreira Alícia, Coelho André, Alvites Rui, Alves Nuno, Geuna Stefano, Maurício Ana Colette
Departamento de Clínicas Veterinárias, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, No. 228, 4050-313 Porto, Portugal.
Centro de Estudos de Ciência Animal (CECA), Instituto de Ciências, Tecnologias e Agroambiente da Universidade do Porto (ICETA), Rua D. Manuel II, Apartado 55142, 4051-401 Porto, Portugal.
Biomedicines. 2023 Jul 25;11(8):2099. doi: 10.3390/biomedicines11082099.
Exosomes have shown promising potential as a therapeutic approach for wound healing. Nevertheless, the translation from experimental studies to commercially available treatments is still lacking. To assess the current state of research in this field, a systematic review was performed involving studies conducted and published over the past five years. A PubMed search was performed for English-language, full-text available papers published from 2018 to June 2023, focusing on exosomes derived from mammalian sources and their application in wound healing, particularly those involving in vivo assays. Out of 531 results, 148 papers were selected for analysis. The findings revealed that exosome-based treatments improve wound healing by increasing angiogenesis, reepithelization, collagen deposition, and decreasing scar formation. Furthermore, there was significant variability in terms of cell sources and types, biomaterials, and administration routes under investigation, indicating the need for further research in this field. Additionally, a comparative examination encompassing diverse cellular origins, types, administration pathways, or biomaterials is imperative. Furthermore, the predominance of rodent-based animal models raises concerns, as there have been limited advancements towards more complex in vivo models and scale-up assays. These constraints underscore the substantial efforts that remain necessary before attaining commercially viable and extensively applicable therapeutic approaches using exosomes.
外泌体已显示出作为伤口愈合治疗方法的巨大潜力。然而，从实验研究到商业可用治疗方法的转化仍然缺乏。为了评估该领域的当前研究状况，进行了一项系统综述，涉及过去五年进行并发表的研究。在PubMed上搜索了2018年至2023年6月发表的英文全文可用论文，重点关注源自哺乳动物的外泌体及其在伤口愈合中的应用，特别是那些涉及体内试验的研究。在531个结果中，选择了148篇论文进行分析。研究结果表明，基于外泌体的治疗方法通过增加血管生成、再上皮化、胶原蛋白沉积和减少瘢痕形成来促进伤口愈合。此外，在所研究的细胞来源和类型、生物材料以及给药途径方面存在显著差异，这表明该领域需要进一步研究。此外，必须对不同的细胞起源、类型、给药途径或生物材料进行比较研究。此外，基于啮齿动物的动物模型占主导地位引发了担忧，因为在向更复杂的体内模型和扩大试验发展方面进展有限。这些限制凸显了在获得使用外泌体的商业可行且广泛适用的治疗方法之前，仍需付出巨大努力。
