Institute of Nutrition and Functional Food (INAF) and Department of Food Sciences, Pavillon Paul-Comtois, Université Laval, Québec, QC G1V 0A6, Canada.
Laboratoire de Transformation Alimentaire et Procédés ElectroMembranaires (LTAPEM, Laboratory of Food Processing and ElectroMembrane Processes), Pavillon Paul Comtois, Université Laval, Québec, QC G1V 0A6, Canada.
Int J Mol Sci. 2023 Aug 19;24(16):12968. doi: 10.3390/ijms241612968.
The development of Metabolic Syndrome (MetS) affects a large number of people around the world and represents a major issue in the field of health. Thus, it is important to implement new strategies to reduce its prevalence, and various approaches are currently under development. Recently, an eco-friendly technology named electrodialysis with ultrafiltration membrane (EDUF) was used successfully for the first time at a semi-industrial scale to produce three fractions concentrated in bioactive peptides (BPs) from an enzymatically hydrolyzed whey protein concentrate (WPC): the initial (F1), the final (F2) and the recovery fraction (F3), and it was demonstrated in vitro that F3 exhibited interesting DPP-IV inhibitory effects. Therefore, the present study aimed to evaluate the effect of each fraction on in vivo models of obesity. A daily dose of 312.5 mg/kg was administered to High Fat/High Sucrose diet (HFHS) induced C57BL6/J mice for eight weeks. The physiological parameters of each group and alterations of their gut microbiota by the fractions were assessed. Little effect of the different fractions was demonstrated on the physiological state of the mice, probably due to the digestion process of the BP content. However, there were changes in the gut microbiota composition and functions of mice treated with F3.
代谢综合征（MetS）的发展影响了全球大量人群，是健康领域的一个主要问题。因此，实施新策略来降低其流行率非常重要，目前正在开发各种方法。最近，一种名为电透析超滤膜（EDUF）的环保技术首次在半工业规模上成功使用，从酶解乳清蛋白浓缩物（WPC）中生产三种富含生物活性肽（BP）的浓缩物：初始（F1）、最终（F2）和回收部分（F3），并在体外证明 F3 具有有趣的 DPP-IV 抑制作用。因此，本研究旨在评估各部分对肥胖体内模型的影响。将 312.5mg/kg 的每日剂量给予高脂肪/高蔗糖饮食（HFHS）诱导的 C57BL6/J 小鼠 8 周。评估了各组的生理参数以及各部分对其肠道微生物群的改变。不同部分对小鼠的生理状态几乎没有影响，这可能是由于 BP 含量的消化过程。然而，用 F3 处理的小鼠的肠道微生物群组成和功能发生了变化。
