Department of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok 65000, Thailand.
Department of Chemistry, Faculty of Science, Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000, Thailand.
Molecules. 2023 Aug 8;28(16):5949. doi: 10.3390/molecules28165949.
Actinobacteria produce a broad spectrum of bioactive substances that are used in the pharmaceutical, agricultural, and biotechnology industries. This study investigates the production of bioactive substances in , isolated from soil under five tropical plants, focusing on their potential as natural antibacterial dyes for silk fabrics. Out of 194 isolates, 44 produced pigments on broken rice as a solid substrate culture. Eight antibacterial pigmented isolates from under (TBRC 15924, TBRC 15927, TBRC 15931), (TBRC 15925, TBRC 15926, TBRC 15928, TBRC 15930), and (TBRC 15929) were studied in more detail. TBRC 15927 was the only isolate where all the crude extracts inhibited the growth of the test organisms, TISTR 518 and DMST 4745. The bioactive compounds present in TBRC 15927 were identified through LC-MS/MS analysis as belonging to the actinomycin group, actinomycin D (or X), X, and X. Also, the ethyl acetate crude extract exhibited non-toxicity at an IC value of 0.029 ± 0.008 µg/mL on the mouse fibroblast L-929 assay. From the 16S rRNA gene sequence analysis, TBRC 15927 had 100% identity with JR-43. Raw silk dyed with the positive antimicrobial TBRC 15927 extract (8.35 mg/mL) had significant (>99.99%) antibacterial properties. TBRC 15927 is the first actinomycin-producing strain reported to grow on broken rice and shows promise for antibacterial silk dyeing.
放线菌产生广泛的生物活性物质，用于制药、农业和生物技术行业。本研究调查了从五种热带植物下的土壤中分离出的 的生物活性物质的产生，重点研究其作为天然抗菌丝织物染料的潜力。在 194 个分离株中，有 44 个在碎米上作为固体基质培养产生色素。从 （TBRC 15924、TBRC 15927、TBRC 15931）、 （TBRC 15925、TBRC 15926、TBRC 15928、TBRC 15930）和 （TBRC 15929）下分离到的 8 个具有抗菌色素的分离株进行了更详细的研究。TBRC 15927 是唯一一种所有粗提取物都能抑制测试生物 （TISTR 518 和 DMST 4745）生长的分离株。通过 LC-MS/MS 分析，TBRC 15927 中存在的生物活性化合物被鉴定为属于放线菌素类，包括放线菌素 D（或 X）、X 和 X。此外，在小鼠成纤维细胞 L-929 测定中，乙酸乙酯粗提取物的 IC 值为 0.029±0.008µg/mL，表现出非毒性。从 16S rRNA 基因序列分析来看，TBRC 15927 与 JR-43 具有 100%的同源性。用具有阳性抗菌活性的 TBRC 15927 提取物（8.35mg/mL）对生丝进行染色，具有显著的（>99.99%）抗菌性能。TBRC 15927 是第一个报道在碎米上生长并显示出用于抗菌丝织物染色潜力的产生放线菌素的菌株。
