Innovation Center of Pesticide Research, Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, China.
Key Laboratory of National Forestry and Grassland Administration on Pest Chemical Control, China Agricultural University, Beijing 100193, China.
Molecules. 2023 Aug 17;28(16):6099. doi: 10.3390/molecules28166099.
Nanotechnology is revolutionizing the efficient production and sustainable development of modern agriculture. Understanding the pesticide activity of both nano- and conventional methods is useful for developing new pesticide formulations. In this study, three solid fluopyram formulations with varying particle sizes were developed, and the mechanisms underlying the difference in the antifungal activity among these formulations were investigated. Wet media milling combined with freeze drying was used to prepare fluopyram nanoparticles (FLU-NS) and a micron-sized solid formulation (FLU-MS), and a jet grinding mill was employed to fabricate fluopyram wettable powder (FLU-WP). The mean particle sizes of FLU-NS, FLU-MS, and FLU-WP were 366.8 nm, 2.99 μm, and 10.16 μm, respectively. Notably, FLU-NS displayed a toxicity index against (gray mold) that was approximately double those of FLU-MS and FLU-WP. Similar trends were noticed in the antifungal tests on . The uptake of FLU-NS by was approximately twice that of FLU-MS and FLU-WP, indicating that fluopyram nanoparticles are more easily taken up by the pathogen (, and display better bioactivity than the larger fluopyram particles. Therefore, the nanosizing of pesticides appears to be a viable strategy to enhance efficiency without increasing the amount of pesticide used.
纳米技术正在彻底改变现代农业的高效生产和可持续发展。了解纳米和常规方法的农药活性对于开发新的农药配方很有用。在这项研究中，开发了三种具有不同粒径的固体氟吡菌酰胺制剂，并研究了这些制剂之间抗真菌活性差异的机制。湿磨法结合冷冻干燥法制备氟吡菌酰胺纳米粒（FLU-NS）和微米级固体制剂（FLU-MS），射流粉碎机制备氟吡菌酰胺可湿性粉剂（FLU-WP）。FLU-NS、FLU-MS 和 FLU-WP 的平均粒径分别为 366.8nm、2.99μm 和 10.16μm。值得注意的是，FLU-NS 对 （灰霉病）的毒性指数约为 FLU-MS 和 FLU-WP 的两倍。在对 的抗真菌试验中也观察到了类似的趋势。FLU-NS 被 的摄取量约为 FLU-MS 和 FLU-WP 的两倍，表明氟吡菌酰胺纳米粒更容易被病原体（和 ）摄取，并且比较大的氟吡菌酰胺颗粒具有更好的生物活性。因此，农药的纳米化似乎是一种提高效率而不增加农药使用量的可行策略。
