Department of Environmental Biology, "Sapienza" University of Rome, P. le Aldo Moro 5, 00185, Rome, Italy.
Department of Chemistry, "Sapienza" University of Rome, 00185, Rome, Italy.
Sci Rep. 2022 May 14;12(1):7989. doi: 10.1038/s41598-022-11533-w.
Botrytis cinerea, responsible for grey mold diseases, is a pathogen with a broad host range, affecting many important agricultural crops, in pre and post harvesting of fruits and vegetables. Commercial fungicides used to control this pathogen are often subjected to photolysis, volatilization, degradation, leaching, and runoff during application. In this context, the use of a delivery system, based on poly (lactic-co-glycolic acid) nanoparticles (PLGA NPs) represents an innovative approach to develop new pesticide formulations to successfully fight B. cinerea infections. In order to study NPs uptake, B. cinerea conidia and mycelium were treated with PLGA NPs loaded with the high fluorescent probe coumarin 6 (Cu6-PLGA NPs) and analyzed under ApoTome fluorescence microscopy. The observations revealed that 50 nm Cu6-PLGA NPs penetrated into B. cinerea conidia and hyphae, as early as 10 min after administration. Pterostilbene, a natural compound, and fluopyram, a synthetic antifungal, were entrapped in PLGA NPs, added to B. cinerea conidia and mycelium, and their antifungal activity was tested. The results revealed that the compounds loaded in NPs exhibited a higher activity against B. cinerea. These results lay the foundations for the use of PLGA NPs as a new strategy in plant pest management.
灰葡萄孢（Botrytis cinerea）是一种宿主范围广泛的病原菌，可导致灰霉病，影响许多重要的农业作物，包括水果和蔬菜的采前和产后。用于控制这种病原菌的商业杀真菌剂在使用过程中经常受到光解、挥发、降解、淋溶和径流的影响。在这种情况下，使用基于聚（乳酸-共-乙醇酸）纳米粒子（PLGA NPs）的递送系统代表了开发新农药制剂的创新方法，可以成功对抗灰葡萄孢感染。为了研究 NPs 的摄取，用负载高荧光探针香豆素 6（Cu6-PLGA NPs）的 PLGA NPs 处理灰葡萄孢分生孢子和菌丝体，并在 ApoTome 荧光显微镜下进行分析。观察结果表明，早在给药后 10 分钟，50nm 的 Cu6-PLGA NPs 就穿透了灰葡萄孢分生孢子和菌丝体。将天然化合物白藜芦醇和合成抗真菌剂氟吡菌胺包封在 PLGA NPs 中，添加到灰葡萄孢分生孢子和菌丝体中，并测试其抗真菌活性。结果表明，负载在 NPs 中的化合物对灰葡萄孢表现出更高的活性。这些结果为将 PLGA NPs 用作植物病虫害管理的新策略奠定了基础。
