Department of Horticulture and Crop Science, The Ohio State University, Wooster, OH 44691.
Department of Plant Pathology, The Ohio State University, Columbus, OH 43210.
Plant Dis. 2020 Jun;104(6):1801-1810. doi: 10.1094/PDIS-10-19-2276-RE. Epub 2020 Apr 14.
infects most major greenhouse crops worldwide. With its increasing resistance to conventional fungicides and the movement of the greenhouse industry toward more sustainable production practices, alternative methods of control are needed. The objective of this study was to evaluate a collection of 60 bacterial strains through both a dual-culture assay and greenhouse trials to identify strains with biocontrol activity against . For the dual-culture assay, each bacterial strain was streaked on potato dextrose agar medium with . The growth reduction and the zone of inhibition were measured. Thirty-five strains reduced the growth of . All strains were also tested in an initial greenhouse trial in which 'Carpet Red Bright' was sprayed and drenched with the bacteria biweekly for 6 weeks. All open flowers were tagged, and plants were inoculated with (1 × 10 conidia per 1 ml). Disease severity indices calculated from the daily flower gray mold severity ratings of all tagged flowers were used to identify the seven top-performing strains. These seven strains were then evaluated in a greenhouse validation trial. The methods were similar to those of the initial greenhouse trials except that replicate numbers were increased. Three strains ( AP54, 14B11, and 89F1) were selected for the ability to reduce infection in a greenhouse production setting. These strains can be used in future studies to develop additional biocontrol products for the management of in floriculture crops.
它感染了全球大多数主要的温室作物。随着其对传统杀菌剂的抗性不断增强，以及温室产业向更可持续的生产实践的发展，需要寻找替代的控制方法。本研究的目的是通过双重培养测定和温室试验来评估 60 株细菌菌株，以鉴定对具有生物防治活性的菌株。在双重培养测定中，将每种细菌菌株划线接种在含有 的马铃薯葡萄糖琼脂培养基上。测量 的生长减少和抑制区。35 株菌株减少了 的生长。所有菌株还在初始温室试验中进行了测试，其中“Carpet Red Bright”每周用细菌喷洒和淋洗两次，持续 6 周。所有开放的花朵都被标记，并用 （每 1 毫升 1 × 10 个分生孢子）接种植物。从所有标记花朵的每日花朵灰霉病严重程度评分中计算出的疾病严重程度指数用于鉴定表现最好的 7 株菌株。然后在温室验证试验中评估这 7 株菌株。方法与初始温室试验相似，只是增加了重复次数。选择了 3 株（AP54、14B11 和 89F1）菌株，因为它们具有在温室生产环境中减少 感染的能力。这些菌株可用于未来的研究，以开发用于花卉作物灰霉病管理的其他生物防治产品。
