Leignadier Julie, Drago Marie, Lesouhaitier Olivier, Barreau Magalie, Dashi Albert, Worsley Oliver, Attia-Vigneau Joan
Lucas Meyer Cosmetics, 195 Route d'Espagne, 31036 Toulouse, France.
Shiseido EMEA, 56A Rue du Faubourg St Honoré, 75008 Paris, France.
Pharmaceutics. 2023 Aug 3;15(8):2083. doi: 10.3390/pharmaceutics15082083.
Acne is a chronic inflammatory skin disease that affects the quality of life of patients. Several treatments exist for acne, but their effectiveness tends to decrease over time due to increasing resistance to treatment and associated side effects. To circumvent these issues, a new approach has emerged that involves combating the pathogen while maintaining the homeostasis of the skin microbiome. Recently, it was shown that the use of a G2 lysine dendrigraft (G2 dendrimer) could specifically decrease the phylotype (IAI) involved in acne, compared to non-acne-causing (phylotype II) bacteria. In the present study, we demonstrate that the efficacy of this technology is related to its 3D structure, which, in contrast to the linear form, significantly decreases the inflammation factor (IL-8) linked to acne. In addition, our in-vitro data confirm the specific activity of the G2 dendrimer: after treatment of bacterial cultures and biofilms, the G2 dendrimer affected neither non-acneic nor commensal bacteria of the skin (, and ). In parallel, comparative in-vitro and in-vivo studies with traditional over-the-counter molecules showed G2's effects on the survival of commensal bacteria and the reduction of acne outbreaks. Finally, metagenomic analysis of the cutaneous microbiota of volunteers who applied a finished cosmetic product containing the G2 dendrimer confirmed the ability of G2 to rebalance cutaneous acne microbiota dysbiosis while maintaining commensal bacteria. These results confirm the value of using this G2 dendrimer to gently prevent the appearance of acne vulgaris while respecting the cutaneous microbiota.
痤疮是一种影响患者生活质量的慢性炎症性皮肤病。针对痤疮有多种治疗方法，但由于对治疗的耐药性增加以及相关副作用，其疗效往往会随着时间的推移而降低。为了解决这些问题，一种新的方法应运而生，该方法涉及在维持皮肤微生物群稳态的同时对抗病原体。最近有研究表明，与非致痤疮（II型菌型）细菌相比，使用G2赖氨酸树枝状聚合物（G2树枝状大分子）可特异性减少参与痤疮形成的菌型（IAI）。在本研究中，我们证明了该技术的疗效与其三维结构有关，与线性形式相比，三维结构可显著降低与痤疮相关的炎症因子（IL-8）。此外，我们的体外数据证实了G2树枝状大分子的特异性活性：在处理细菌培养物和生物膜后，G2树枝状大分子对皮肤的非痤疮性细菌和共生细菌均无影响（分别为、和）。同时，与传统非处方分子进行的体外和体内比较研究表明，G2对共生细菌的存活以及痤疮爆发的减少有影响。最后，对使用含有G2树枝状大分子的成品化妆品的志愿者皮肤微生物群进行宏基因组分析，证实了G2在维持共生细菌的同时重新平衡皮肤痤疮微生物群失调的能力。这些结果证实了使用这种G2树枝状大分子在尊重皮肤微生物群的同时温和预防寻常痤疮出现的价值。
