Department of Biotechnology, Middle East Technical University, Ankara, Turkiye.
Biotechnology Institute, Ankara University, Ankara, Turkiye.
Pathol Res Pract. 2023 Sep;249:154780. doi: 10.1016/j.prp.2023.154780. Epub 2023 Aug 22.
Breast cancer has been among the most prominent cancers with high mortality. Currently most of the offered therapeutics are toxic; hence, less toxic therapeutic intervention is required. Here, we studied the molecular mechanisms of the effect of a phytoestrogen Emodin on estrogen receptor positive MCF-7 and negative MDA-MB-231 cells by carrying out a comprehensive network assessment. Differentially expressed microRNAs along with their previously identified differentially expressed mRNAs were analyzed through microarrays by using integrative systems biology approach. For each cell line miRNA-target gene networks were built, gene ontology and pathway enrichment analyses were performed, enrichment maps were constructed and the potential key genes, miRNAs and miRNA-gene interactions were studied.
乳腺癌一直是死亡率较高的突出癌症之一。目前，大多数提供的治疗方法都是有毒的，因此需要毒性较小的治疗干预。在这里，我们通过进行全面的网络评估，研究了植物雌激素大黄素对雌激素受体阳性 MCF-7 和阴性 MDA-MB-231 细胞作用的分子机制。通过整合系统生物学方法，使用微阵列分析了差异表达的 microRNAs 及其先前鉴定的差异表达的 mRNAs。为每条细胞系构建了 miRNA-靶基因网络，进行了基因本体论和途径富集分析，构建了富集图谱，并研究了潜在的关键基因、miRNAs 和 miRNA-基因相互作用。
