Barulin Aleksandr, Park Hyemi, Park Byullee, Kim Inki
Department of Biophysics, Institute of Quantum Biophysics, Sungkyunkwan University, Suwon 16419, Republic of Korea.
Department of Intelligent Precision Healthcare Convergence, Sungkyunkwan University, Suwon 16419, Republic of Korea.
Photoacoustics. 2023 Aug 16;32:100545. doi: 10.1016/j.pacs.2023.100545. eCollection 2023 Aug.
Photoacoustic microscopy is advancing with research on utilizing ultraviolet and visible light. Dual-wavelength approaches are sought for observing DNA/RNA- and vascular-related disorders. However, the availability of high numerical aperture lenses covering both ultraviolet and visible wavelengths is severely limited due to challenges such as chromatic aberration in the optics. Herein, we present a groundbreaking proposal as a pioneering simulation study for incorporating multilayer metalenses into ultraviolet-visible photoacoustic microscopy. The proposed metalens has a thickness of 1.4 µm and high numerical aperture of 0.8. By arranging cylindrical hafnium oxide nanopillars, we design an achromatic transmissive lens for 266 and 532 nm wavelengths. The metalens achieves a diffraction-limited focal spot, surpassing commercially available objective lenses. Through three-dimensional photoacoustic simulation, we demonstrate high-resolution imaging with superior endogenous contrast of targets with ultraviolet and visible optical absorption bands. This metalens will open new possibilities for downsized multispectral photoacoustic microscopy in clinical and preclinical applications.
光声显微镜随着利用紫外光和可见光的研究而不断发展。人们正在寻求双波长方法来观察与DNA/RNA和血管相关的疾病。然而，由于光学系统中的色差等挑战，同时覆盖紫外和可见波长的高数值孔径透镜的可用性受到严重限制。在此，我们提出一项开创性的提议，作为将多层超构透镜纳入紫外-可见光照声显微镜的开创性模拟研究。所提出的超构透镜厚度为1.4微米，数值孔径高达0.8。通过排列圆柱形氧化铪纳米柱，我们设计了一种用于266纳米和532纳米波长的消色差透射透镜。该超构透镜实现了衍射极限焦斑，优于市售物镜。通过三维光声模拟，我们展示了对具有紫外和可见光学吸收带的目标进行具有卓越内源性对比度的高分辨率成像。这种超构透镜将为临床和临床前应用中的小型化多光谱光声显微镜开辟新的可能性。
