Shi Jun, Ma DongXue, Gao ShanHu, Long Fei, Wang Xin, Pu XingYu, Cannon Richard D, Han Ting-Li
State Key Laboratory of Ultrasound in Medicine and Engineering, College of Biomedical Engineering, Chongqing Medical University, Chongqing, China.
Chongqing Key Laboratory of Biomedical Engineering, Chongqing Medical University, Chongqing, China.
Front Microbiol. 2023 Aug 15;14:1219763. doi: 10.3389/fmicb.2023.1219763. eCollection 2023.
Obesity and diabetes are common chronic metabolic disorders which can cause an imbalance of the intestinal flora and gut-liver metabolism. Several studies have shown that probiotics, including Nissle 1917 (EcN), promote microbial balance and metabolic health. However, there are no studies on how EcN outer membrane vesicles (EcN-OMVs) influence the intestinal microflora and affect the metabolic disorders of obesity and diabetes.
In this study, we evaluated the effects of EcN-OMVs on high-fat diet (HFD)-induced obesity and HFD + streptozotocin (STZ)-induced diabetes.
EcN-OMVs could reduce body weight, decrease blood glucose, and increase plasma insulin in obese mice. Similarly, EcN-OMVs treatment could modify the ratio of / in the gut, elevate intestinal short-chain fatty acid (SCFA)-producing flora, and influence the SCFA content of the intestine. Furthermore, the intestinal metabolites ornithine and fumaric acid, hepatic ω-6 unsaturated fatty acids, and SCFAs were significantly increased after administering EcN-OMVs.
Overall, this study showed that EcN-OMVs might act as post-biotic agents that could modulate gut-liver metabolism and ameliorate the pathophysiology of obesity and diabetes.
肥胖和糖尿病是常见的慢性代谢紊乱疾病，可导致肠道菌群和肠肝代谢失衡。多项研究表明，包括Nissle 1917（EcN）在内的益生菌可促进微生物平衡和代谢健康。然而，关于EcN外膜囊泡（EcN-OMVs）如何影响肠道微生物群并影响肥胖和糖尿病的代谢紊乱，尚无相关研究。
在本研究中，我们评估了EcN-OMVs对高脂饮食（HFD）诱导的肥胖和HFD+链脲佐菌素（STZ）诱导的糖尿病的影响。
EcN-OMVs可降低肥胖小鼠的体重、血糖，并增加血浆胰岛素水平。同样，EcN-OMVs治疗可改变肠道中/的比例，增加肠道产生短链脂肪酸（SCFA）的菌群，并影响肠道SCFA含量。此外，给予EcN-OMVs后，肠道代谢产物鸟氨酸和富马酸、肝脏ω-6不饱和脂肪酸和SCFAs显著增加。
总体而言，本研究表明EcN-OMVs可能作为后生元制剂，调节肠肝代谢，改善肥胖和糖尿病的病理生理过程。
