Galarza-Verkovitch Denisse, Turak Onur, Wiese Jutta, Rahn Tanja, Hentschel Ute, Borchert Erik
GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel, Schleswig-Holstein, Germany.
Christian-Albrechts University of Kiel, Kiel, Schleswig-Holstein, Germany.
AIMS Microbiol. 2023 Jun 15;9(3):518-539. doi: 10.3934/microbiol.2023027. eCollection 2023.
Plastics have quickly become an integral part of modern life. Due to excessive production and improper waste disposal, they are recognized as contaminants present in practically all habitat types. Although there are several polymers, polyethylene terephthalate (PET) is of particular concern due to its abundance in the environment. There is a need for a solution that is both cost-effective and ecologically friendly to address this pollutant. The use of microbial depolymerizing enzymes could offer a biological avenue for plastic degradation, though the full potential of these enzymes is yet to be uncovered. The purpose of this study was to use (1) plate-based screening methods to investigate the plastic degradation potential of marine bacteria from the order Enterobacterales collected from various organismal and environmental sources, and (2) perform genome-based analysis to identify polyesterases potentially related to PET degradation. 126 bacterial isolates were obtained from the strain collection of RD3, Research Unit Marine Symbioses-GEOMAR-and sequentially tested for esterase and polyesterase activity, in combination here referred to as PETase-like activity. The results show that members of the microbial families , , and , derived from marine sponges and bryozoans, are the most promising candidates within the order Enterobacterales. Furthermore, 389 putative hydrolases from the α/β superfamily were identified in 23 analyzed genomes, of which 22 were sequenced for this study. Several candidates showed similarities with known PETases, indicating underlying enzymatic potential within the order Enterobacterales for PET degradation.
塑料已迅速成为现代生活中不可或缺的一部分。由于过度生产和不当的废物处理，它们被认为是几乎所有栖息地类型中都存在的污染物。尽管有几种聚合物，但聚对苯二甲酸乙二酯（PET）因其在环境中的大量存在而备受关注。需要一种既经济高效又生态友好的解决方案来解决这种污染物问题。使用微生物解聚酶可以为塑料降解提供一条生物学途径，不过这些酶的全部潜力尚未被发掘。本研究的目的是：（1）使用基于平板的筛选方法来研究从各种生物和环境来源收集的肠杆菌目海洋细菌的塑料降解潜力；（2）进行基于基因组的分析，以鉴定可能与PET降解相关的聚酯酶。从海洋共生研究组 - 亥姆霍兹海洋研究中心（GEOMAR）的RD3菌株库中获得了126株细菌分离物，并依次测试其酯酶和聚酯酶活性，在此将二者合称为类PET酶活性。结果表明，源自海洋海绵和苔藓虫的微生物科、和的成员是肠杆菌目中最有潜力的候选者。此外，在23个分析的基因组中鉴定出了389个来自α/β超家族的假定水解酶，其中22个是为本研究测序的。几个候选者与已知的PET酶具有相似性，表明肠杆菌目内存在潜在的PET降解酶活性。
