一种用于血型血清学中高质量冰冻红细胞的简单方法。
A simple method for high-quality frozen red cells in blood group serology.
作者信息
Högman C F, Hornblower M L, Flodin M, Gillberg G, Meryman H T, Säfwenberg J
出版信息
Transfusion. 1986 Sep-Oct;26(5):434-6. doi: 10.1046/j.1537-2995.1986.26587020120.x.
Abstract
A high-glycerol method for long-term storage of red cells for use in blood group serology testing is described. Total freezing-thawing-washing hemolysis was 8.05 +/- 0.3 percent. Enzyme treatment could be performed before freezing or after reconstitution. The cells performed well with manual and automated techniques. No changes in agglutinability were noted during 10 days of liquid storage at 4 degrees C following thawing.
摘要
本文描述了一种用于血型血清学检测的红细胞长期保存的高甘油方法。总冻融洗涤溶血率为8.05±0.3%。酶处理可在冷冻前或复溶后进行。这些细胞在手工和自动化技术操作中表现良好。解冻后在4℃液体保存10天期间未观察到凝集性变化。
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