Beaujean F, Le Forestier C, Duedari N
Rev Fr Transfus Immunohematol. 1982 Sep;25(4):427-38. doi: 10.1016/s0338-4535(82)80032-9.
A method of red cell preservation by freezing at -25 degrees C is described. Glycerol is added to the red blood cells in the primary polyvinyl chloride plastic collection bag to achieve a concentration of 28 pr cent (W/V). The blood cells are concentrated by centrifugation and the supernatant glycerol is discarded. Glycerolized red cells are frozen and stored at -25 degrees C for 1 to 6 months. After thawing, Sodium chloride solutions are used to wash the red cells in the IBM Blood Processor 2991. The following parameters have been investigated before freezing, after thawing and washing and after storage of red blood cells at 4 degrees C for 24 hours: --Hemoglobin level --leukocytes and platelets --amount of 2-3 DPG and ATP. Preliminary data show that the in vitro quality of erythrocytes stored at -25 degrees C is well preserved for 4 months and that this simple method can be applied to blood preservation in any Blood Center.
描述了一种在-25℃冷冻保存红细胞的方法。在初始的聚氯乙烯塑料采血袋中向红细胞添加甘油，使其浓度达到28%（重量/体积）。通过离心浓缩血细胞，弃去上清甘油。甘油化红细胞被冷冻并在-25℃储存1至6个月。解冻后，使用氯化钠溶液在IBM血液处理器2991中洗涤红细胞。在冷冻前、解冻和洗涤后以及红细胞在4℃储存24小时后，对以下参数进行了研究：--血红蛋白水平--白细胞和血小板--2,3-二磷酸甘油酸（2,3-DPG）和三磷酸腺苷（ATP）的含量。初步数据表明，在-25℃储存的红细胞的体外质量在4个月内保存良好，并且这种简单方法可应用于任何血液中心的血液保存。
