School and Hospital of Stomatology, Tianjin Medical University, Tianjin, 300070, China.
Department of stomatology, Economic and Technological Development Zone, No.7 people's hospital of Zhengzhou, No. 17, Jingnan 5th Road, Zhengzhou City, Henan Province, 450003, China.
J Mater Sci Mater Med. 2023 Sep 2;34(9):45. doi: 10.1007/s10856-023-06745-z.
The aim of this study is to investigate a robust and stable calcium-phosphorus system to remineralize human early enamel caries lesions with nanocomplexes of carboxymethyl chitosan/L-serine/amorphous calcium phosphate (CMC-Ser-ACP) to develop an effective method for mimicking the amelogenin (AMEL) mineralization pattern through ACP assembly. A CMC-Ser-ACP nanocomplex solution was first synthesized by a chemical precipitation method, and then 1% sodium hypochlorite (NaClO) was added to induce ACP phase formation. The morphologies of the nanocomplexes were characterized by transmission electron microscopy (TEM), and zeta potential analysis and Fourier transform infrared spectroscopy (FTIR) were performed to detect surface charge and functional group changes. The subtle changes of the demineralized enamel models induced by the remineralization effect were observed by scanning electron microscopy (SEM) and X-ray diffraction (XRD). The CMC-Ser-ACP nanocomplex solution could be preserved without any precipitation for 45 days. After the application of NaClO and through the guidance of Ser, ACP nanoparticles transformed into relatively orderly arranged hydroxyapatite (HAP) crystals, generating an aprismatic enamel-like layer closely integrated with the demineralized enamel, which resulted in enhanced mechanical properties for the treatment of early enamel caries lesions. The CMC-Ser-ACP nanocomplex solution is a remineralization system with great solution stability, and when NaClO is added, it can rapidly regenerate an aprismatic enamel-like layer in situ on the demineralized enamel surface. This novel remineralization system has stable chemical properties and can greatly increase the therapeutic effects against early enamel caries.
本研究旨在探索一种稳定且坚固的钙磷体系，以利用羧甲基壳聚糖/L-丝氨酸/无定形磷酸钙（CMC-Ser-ACP）纳米复合物来对早期釉质龋损进行再矿化，从而开发一种有效方法来模拟釉原蛋白（AMEL）的矿化模式，通过 ACP 组装。首先通过化学沉淀法合成 CMC-Ser-ACP 纳米复合物溶液，然后加入 1%次氯酸钠（NaClO）诱导 ACP 相形成。通过透射电子显微镜（TEM）对纳米复合物的形态进行了表征，并进行了zeta 电位分析和傅里叶变换红外光谱（FTIR）检测，以检测表面电荷和官能团的变化。通过扫描电子显微镜（SEM）和 X 射线衍射（XRD）观察脱矿釉质模型在再矿化作用下的细微变化。CMC-Ser-ACP 纳米复合物溶液在没有任何沉淀的情况下可以保存 45 天。在 NaClO 的应用下，通过 Ser 的引导，ACP 纳米颗粒转化为相对有序排列的羟基磷灰石（HAP）晶体，生成与脱矿釉质紧密结合的无定形釉质样层，从而增强了治疗早期釉质龋损的机械性能。CMC-Ser-ACP 纳米复合物溶液是一种具有出色溶液稳定性的再矿化体系，加入 NaClO 后，可迅速在脱矿釉质表面原位再生无定形釉质样层。这种新型再矿化体系具有稳定的化学性质，可以大大提高针对早期釉质龋的治疗效果。
