Azi Fidelis, Wang Zhe, Chen Wenhao, Lin Dewei, Xu Peng
Department of Chemical Engineering, Guangdong Technion-Israel Institute of Technology (GTIIT), Shantou, Guangdong 515063, China; Technion-Israel Institute of Technology, Haifa 3200002, Israel.
Institute of Agro-Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Trends Biotechnol. 2024 Feb;42(2):197-211. doi: 10.1016/j.tibtech.2023.07.008. Epub 2023 Aug 31.
Ganoderma lucidum holds a colossal reservoir of hydrolytic enzymes and therapeutic compounds and can be a sustainable source of proteins and bioactive compounds. Its metabolic versatility, propelled by its rich genome content, provides excellent biosynthetic machinery for innovation-driven pathway engineering. However, robust regulatory networks and low frequency of homologous recombination are critical bottlenecks that limit the development of molecular tools and precise genetic markers for biomanufacturing innovations in this organism. Modern synthetic biology provides tools that could help to accelerate precise multiple gene targeting and editing and untangling the biosynthetic machinery of G. lucidum. This review provides insight into molecular strategies to unwind the regulatory bottlenecks and transform G. lucidum into efficient cell factories for food and nutraceuticals.
灵芝含有大量的水解酶和治疗性化合物，并且可以成为蛋白质和生物活性化合物的可持续来源。其丰富的基因组含量推动了它的代谢多样性，为创新驱动的途径工程提供了出色的生物合成机制。然而，强大的调控网络和同源重组的低频率是关键瓶颈，限制了该生物体在生物制造创新方面分子工具和精确遗传标记的开发。现代合成生物学提供的工具有助于加速精确的多基因靶向和编辑，并解开灵芝的生物合成机制。本综述深入探讨了消除调控瓶颈并将灵芝转化为食品和营养保健品高效细胞工厂的分子策略。
