Guangxi Key Laboratory of Rare and Endangered Animal Ecology, Guangxi Normal University, Guilin, China.
Institute of Tumor Prevention and Control, People's Hospital of Yangzhong City, Yangzhong, China.
Front Cell Infect Microbiol. 2023 Aug 17;13:1221433. doi: 10.3389/fcimb.2023.1221433. eCollection 2023.
The present study aims to investigate the effect of (Hp) infection on gastric mucosal microbiota in patients with chronic gastritis.
Here recruited a population of 193 patients with both chronic gastritis and positive rapid urease, including 124 patients with chronic atrophic gastritis (CAG) and 69 patients with chronic non-atrophic gastritis (nCAG). Immunoblotting was used to detect four serum Hp antibodies (UreA, UreB, VacA and CagA) to determine the types of virulent Hp-I and avirulent Hp-II infections. Gastric microbiota was profiled by 16S rRNA gene V3-V4 region, and R software was used to present the relationship between the microbial characteristics and the type of Hp infection.
In the stomach of patients with Hp-positive gastritis, the dominant gastric bacterial genera included (23.94%), (20.28%), (9.99%), (9.21%), (5.05%), and (4.75%). The proportion of Hp-I infection was significantly higher in CAG patients (91.1%) than in nCAG patients (71.0%) ( < 0.001). The gastric microbiota richness index (observed OTUs, Chao) was significantly lower in CAG patients than in nCAG patients (0.05). Compared with avirulent Hp-II infection, virulent Hp-I infection significantly decreased the Shannon index in CAG patients (0.05). In nCAG patients, Hp-I infected patients had lower abundances of several dominant gastric bacteria (, , , , ) than Hp-II infected patients. Meanwhile, in CAG patients, Hp-I infected patients occupied lower abundances of several dominant oral bacteria (, and ) than Hp-II infected patients. In addition, bile reflux significantly promoted the colonization of dominant oral microbiota (, and ) in the stomach of CAG patients. There was no significant symbiotic relationship between bacteria and non- bacteria in the stomach of nCAG patients, while bacteria distinctly linked with the non- bacteria (, , , and ) in CAG patients.
Virulent Hp infection alters the gastric microbiota, reduces microbial diversity, and enhances the symbiotic relationship between the bacteria and non- bacteria in patients with chronic gastritis. The data provides new evidence for treating Hp infection by improving the gastric microbiota.
本研究旨在探讨幽门螺杆菌（Hp）感染对慢性胃炎患者胃黏膜微生物群的影响。
本研究招募了 193 名同时患有慢性胃炎和快速尿素酶阳性的患者，其中包括 124 名慢性萎缩性胃炎（CAG）患者和 69 名慢性非萎缩性胃炎（nCAG）患者。采用免疫印迹法检测四种血清 Hp 抗体（UreA、UreB、VacA 和 CagA），以确定毒力 Hp-I 和非毒力 Hp-II 感染的类型。通过 16S rRNA 基因 V3-V4 区对胃微生物群进行分析，使用 R 软件呈现微生物特征与 Hp 感染类型之间的关系。
在 Hp 阳性胃炎患者的胃中，优势胃细菌属包括（23.94%）、（20.28%）、（9.99%）、（9.21%）、（5.05%）和（4.75%）。CAG 患者 Hp-I 感染的比例（91.1%）明显高于 nCAG 患者（71.0%）（<0.001）。CAG 患者的胃微生物丰富度指数（观察到的 OTUs，Chao）明显低于 nCAG 患者（0.05）。与非毒力 Hp-II 感染相比，毒力 Hp-I 感染显著降低了 CAG 患者的 Shannon 指数（0.05）。在 nCAG 患者中，与 Hp-II 感染患者相比，Hp-I 感染患者几种优势胃细菌（、、、、）的丰度较低。同时，在 CAG 患者中，Hp-I 感染患者几种优势口腔细菌（、和）的丰度低于 Hp-II 感染患者。此外，胆汁反流显著促进了 CAG 患者胃中优势口腔微生物群（、和）的定植。在 nCAG 患者的胃中，细菌与非细菌之间没有明显的共生关系，而细菌与非细菌（、、、和）在 CAG 患者中明显相关。
毒力 Hp 感染改变了胃微生物群，降低了微生物多样性，并增强了慢性 gastritis 患者中细菌与非细菌之间的共生关系。该数据为通过改善胃微生物群来治疗 Hp 感染提供了新的证据。
