Liu Qingyan, Sun Shuo, Yang Zhengwei, Shao Yan, Li Xiaorong
Tianjin Key Laboratory of Retinal Functions and Diseases, Tianjin Branch of National Clinical Research Center for Ocular Disease, Eye Institute and School of Optometry, Tianjin Medical University Eye Hospital, Tianjin, 300384, People's Republic of China.
Department of Ophthalmology, Anhui NO.2 Provincial People's hospital, Hefei, 230041, People's Republic of China.
J Inflamm Res. 2023 Aug 29;16:3783-3797. doi: 10.2147/JIR.S417791. eCollection 2023.
Neovascular age-related macular degeneration (nAMD) and its subtype, polypoidal choroidal vasculopathy (PCV), are common choroidal vasculopathies. Although they share many common clinical manifestations and treatment strategies, a lack of comprehensive analysis of these conditions means that it is difficult for researchers to further explore the common pathomechanisms of nAMD and PCV. The aim of this study was to characterize aqueous humor (AH) proteome alterations and identify a novel biomarker related to both nAMD and PCV.
Liquid Chromatography with tandem mass spectrometry (LC-MS/MS) was adopted to analyze the AH proteomes of nAMD, PCV and controls. The target protein was validated using the enzyme-linked immunosorbent assay (ELISA) and subjected to receiver operating characteristic (ROC) curve analysis.
A total of 737 different proteins were identified in all the groups, of which 544 were quantifiable. The bioinformatics analysis suggested that immune response activation is the essential event in both nAMD and PCV. Serum amyloid A (SAA) 4 is closely associated with a number of chronic inflammatory diseases, and it was enriched as the hub protein. ROC analysis showed that SAA4 could distinguish both nAMD and PCV from the controls.
This comprehensive study provides insights into, and furthers our understanding of, the pathological mechanism of nAMD and PCV. Additionally, the SAA4 level alteration may serve as a common biomarker of nAMD and PCV.
新生血管性年龄相关性黄斑变性（nAMD）及其亚型息肉状脉络膜血管病变（PCV）是常见的脉络膜血管病变。尽管它们有许多共同的临床表现和治疗策略，但缺乏对这些病症的全面分析意味着研究人员难以进一步探索nAMD和PCV的共同发病机制。本研究的目的是表征房水（AH）蛋白质组的变化，并鉴定一种与nAMD和PCV均相关的新型生物标志物。
采用液相色谱串联质谱（LC-MS/MS）分析nAMD、PCV患者及对照组的房水蛋白质组。使用酶联免疫吸附测定（ELISA）对目标蛋白进行验证，并进行受试者工作特征（ROC）曲线分析。
所有组中共鉴定出737种不同的蛋白质，其中544种可定量。生物信息学分析表明，免疫反应激活是nAMD和PCV的关键事件。血清淀粉样蛋白A（SAA）4与多种慢性炎症性疾病密切相关，并且它作为枢纽蛋白富集。ROC分析表明，SAA4可以将nAMD和PCV与对照组区分开来。
这项综合研究为nAMD和PCV的病理机制提供了见解，并加深了我们对其的理解。此外，SAA4水平的改变可能作为nAMD和PCV的共同生物标志物。
