Mao Jianbo, Chen Nuo, Zhang Shian, Fang Yuyan, Zheng Zicheng, Wu Sulan, Ye Xin, Chen Yijing, Chen Yiqi, Shen Lijun
Center for Rehabilitation Medicine, Department of Ophthalmology, Zhejiang Provincial People's Hospital, Hangzhou, China.
School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, China.
Front Med (Lausanne). 2022 Sep 23;9:973025. doi: 10.3389/fmed.2022.973025. eCollection 2022.
To investigate the associations between cytokine levels in the aqueous humor (AH) and hyperreflective foci (HF) on spectral-domain optical coherence tomography (SD-OCT) in neovascular age-related macular degeneration (nAMD) and polypoidal choroidal vasculopathy (PCV).
The prospective study included 63 eyes with nAMD, 44 with PCV, and 43 with cataracts (Controls). AH samples were obtained before anti-vascular endothelial growth factor (VEGF) therapy or cataract surgery. Cytokines interleukin 6 (IL-6), IL-8, IL-10, interferon-inducible protein 10 (IP-10), monocyte chemotactic protein 1 (MCP-1), and VEGF were measured by multiplex bead assay. Best-corrected visual acuity (BCVA), central macular thickness (CMT), and the number of HF were evaluated at baseline and 1 month after anti-VEGF treatment.
No significances difference in IL-6 and IL-8 levels were noted among the three groups ( = 0.370 and = 0.067). VEGF, IP-10, and IL-10 levels were significantly higher in nAMD and PCV groups than in Controls (all < 0.05). In nAMD, HF was positively correlated with VEGF ( = 0.300, = 0.025) and in eyes with HF group, VEGF and IL-10 were significantly higher than those without HF ( = 0.008 and = 0.022). In PCV, no correlation was observed between HF and cytokines (all > 0.05). After anti-VEGF treatment, patients with HF in nAMD and PCV were predisposed to worse visual outcomes ( = 0.022 and = 0.015) and a significantly greater reduction in CMT ( = 0.001 and = 0.057). And nAMD patients with HF were more sensitive to anti-VEGF treatment than those without HF ( = 0.029).
In the nAMD group, HF was positively correlated with VEGF. Patients in nAMD with HF had elevated levels of VEGF and IL-10 and responded favorably to anti-VEGF. HF might serve as an inflammatory biomarker and a predictive factor for therapeutic efficacy in patients with nAMD.
研究新生血管性年龄相关性黄斑变性（nAMD）和息肉状脉络膜血管病变（PCV）患者房水（AH）中细胞因子水平与光谱域光学相干断层扫描（SD - OCT）上高反射灶（HF）之间的关联。
这项前瞻性研究纳入了63只患nAMD的眼睛、44只患PCV的眼睛以及43只患白内障的眼睛（对照组）。在抗血管内皮生长因子（VEGF）治疗或白内障手术前采集AH样本。通过多重微珠分析法检测细胞因子白细胞介素6（IL - 6）、IL - 8、IL - 10、干扰素诱导蛋白10（IP - 10）、单核细胞趋化蛋白1（MCP - 1）和VEGF。在基线以及抗VEGF治疗1个月后评估最佳矫正视力（BCVA）、中心黄斑厚度（CMT）和HF数量。
三组之间IL - 6和IL - 8水平无显著差异（P = 0.370和P = 0.067）。nAMD组和PCV组的VEGF、IP - 10和IL - 10水平显著高于对照组（均P < 0.05）。在nAMD中，HF与VEGF呈正相关（r = 0.300，P = 0.025），在有HF组的眼睛中，VEGF和IL - 10显著高于无HF组（P = 0.008和P = 0.022）。在PCV中，未观察到HF与细胞因子之间的相关性（均P > 0.05）。抗VEGF治疗后，nAMD和PCV中有HF的患者更容易出现较差的视力结果（P = 0.022和P = 0.015），并且CMT显著降低更多（P = 0.001和P = 0.057）。而且nAMD中有HF的患者比无HF的患者对抗VEGF治疗更敏感（P = 0.029）。
在nAMD组中，HF与VEGF呈正相关。nAMD中有HF的患者VEGF和IL - 10水平升高，对抗VEGF反应良好。HF可能作为nAMD患者的一种炎症生物标志物和治疗疗效的预测因素。
