SlWRKY57-SlVQ21/SlVQ16 模块调控番茄的盐胁迫反应。

The SlWRKY57-SlVQ21/SlVQ16 module regulates salt stress in tomato.

机构信息

Plant Science and Technology College, Beijing University of Agriculture, Beijing, 102206, China.

Beijing Key Laboratory for Agricultural Application and New Technique, Beijing University of Agriculture, Beijing, 102206, China.

出版信息

J Integr Plant Biol. 2023 Nov;65(11):2437-2455. doi: 10.1111/jipb.13562. Epub 2023 Oct 4.

DOI:10.1111/jipb.13562

PMID:37665103

Abstract

Salt stress is a major abiotic stress which severely hinders crop production. However, the regulatory network controlling tomato resistance to salt remains unclear. Here, we found that the tomato WRKY transcription factor WRKY57 acted as a negative regulator in salt stress response by directly attenuating the transcription of salt-responsive genes (SlRD29B and SlDREB2) and an ion homeostasis gene (SlSOS1). We further identified two VQ-motif containing proteins SlVQ16 and SlVQ21 as SlWRKY57-interacting proteins. SlVQ16 positively, while SlVQ21 negatively modulated tomato resistance to salt stress. SlVQ16 and SlVQ21 competitively interacted with SlWRKY57 and antagonistically regulated the transcriptional repression activity of SlWRKY57. Additionally, the SlWRKY57-SlVQ21/SlVQ16 module was involved in the pathway of phytohormone jasmonates (JAs) by interacting with JA repressors JA-ZIM domain (JAZ) proteins. These results provide new insights into how the SlWRKY57-SlVQ21/SlVQ16 module finely tunes tomato salt tolerance.

摘要

盐胁迫是一种严重影响作物生产的非生物胁迫。然而，控制番茄耐盐性的调控网络仍不清楚。在这里，我们发现番茄 WRKY 转录因子 WRKY57 通过直接减弱盐响应基因（SlRD29B 和 SlDREB2）和离子稳态基因（SlSOS1）的转录，作为盐胁迫响应的负调控因子起作用。我们进一步鉴定了两个含有 VQ 基序的蛋白 SlVQ16 和 SlVQ21 作为 SlWRKY57 的互作蛋白。SlVQ16 正向，而 SlVQ21 负向调节番茄耐盐性。SlVQ16 和 SlVQ21 与 SlWRKY57 竞争相互作用，并拮抗调节 SlWRKY57 的转录抑制活性。此外，SlWRKY57-SlVQ21/SlVQ16 模块通过与茉莉酸（JAs）的抑制因子 JA-ZIM 结构域（JAZ）蛋白相互作用，参与了植物激素途径。这些结果为 SlWRKY57-SlVQ21/SlVQ16 模块如何精细调节番茄耐盐性提供了新的见解。