Li Xin, Liu Xiyan, Pan Feng, Hu Junling, Han Yunhao, Bi Ripu, Zhang Chen, Liu Yan, Wang Yong, Liang Zengwen, Zhu Can, Guo Yanmei, Huang Zejun, Wang Xiaoxuan, Du Yongchen, Liu Lei, Li Junming
State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Huhhot, 010031, China.
BMC Genomics. 2024 Dec 3;25(1):1170. doi: 10.1186/s12864-024-11101-8.
As two of the most impactful abiotic stresses, salt and drought strongly affect tomato growth and development, especially at the seedling stage. However, dissection of the genetic basis underlying salt/drought tolerance at seedling stage in tomato remains limited in scope.
Here, we reported an analysis of major quantitative trait locus (QTL) and potential causal genetic variations in seedling stage salt/drought tolerance in recombinant inbred lines (n = 201) of S. pimpinellifolium and S. lycopersicum parents by whole genome resequencing. A total of 5 QTLs on chromosome 1, 3, 5, 7 and 12 for salt tolerance (ST) and 15 QTLs on chromosome 1, 3, 4, 8, 9, 10, 12 for drought tolerance (DT) were identified by linkage mapping. The proportion of phenotypic variation explained (PVE%) by these QTLs ranged from 4.91 to 15.86. Two major QTLs qST7 and qDT1-3 were detected in both two years, for which two candidate genes (methionine sulfoxide reductase SlMSRB1 and brassinosteroid insensitive 1-like receptor SlBRL1) and the potential functional variations were further analyzed. Taking advantage of the tomato population resequencing data, the frequency changes of the potential favorable QTL allele for seedling stage ST/DT during tomato breeding were explored.
These results will be beneficial for the exploration of salt/drought tolerance genes at seedling stages, laying a foundation for marker-assisted breeding for seedling stage salt/drought tolerance.
盐胁迫和干旱胁迫作为两种影响最大的非生物胁迫，对番茄的生长发育有强烈影响，尤其是在幼苗期。然而，番茄幼苗期耐盐/耐旱遗传基础的解析范围仍然有限。
在此，我们通过全基因组重测序，报道了对潘那利番茄和栽培番茄亲本的重组自交系（n = 201）幼苗期耐盐/耐旱主要数量性状位点（QTL）及潜在因果遗传变异的分析。通过连锁图谱鉴定出1号、3号、5号、7号和12号染色体上共5个耐盐（ST）QTL，以及1号、3号、4号、8号、9号、10号、12号染色体上15个耐旱（DT）QTL。这些QTL解释的表型变异比例（PVE%）在4.91至15.86之间。两年均检测到两个主要QTL，即qST7和qDT1-3，并对其两个候选基因（甲硫氨酸亚砜还原酶SlMSRB1和油菜素内酯不敏感1样受体SlBRL1）及潜在功能变异进行了进一步分析。利用番茄群体重测序数据，探究了番茄育种过程中幼苗期ST/DT潜在有利QTL等位基因的频率变化。
这些结果将有助于探索幼苗期耐盐/耐旱基因，为幼苗期耐盐/耐旱分子标记辅助育种奠定基础。
