Ferro M E, Galmarini M, Riera C M
Am J Reprod Immunol Microbiol. 1985 Nov;9(3):73-6. doi: 10.1111/j.1600-0897.1985.tb00280.x.
Pretreatment of rats with low doses of purified fraction of rat male accessory glands (containing the autoantigen) markedly reduced the immune response to autoantigen when animals were subsequently challenged with modified rat male accessory glands in complete Freund's adjuvant. The rats pretreated with low doses of antigen prior to immunization showed marked suppression of delayed-type hypersensitivity reaction (P less than 0.001) when compared to control animals (pretreated with rat lung saline extract or 0.15 M NaCl). There was also enhancement of migration of macrophages in test male rats, whereas the migration was inhibited in control male rats (P less than 0.01). The stimulation of migration found in the male rats in which the response was inhibited would suggest the presence of a migration stimulation factor, which is considered a marker of suppressor cell activity. Humoral immunity was also reduced. Pretreatment of rats with low doses of the antigen markedly reduced the immune response, probably because of the induction of suppressor cells.
用低剂量纯化的大鼠雄性附属性腺提取物（含自身抗原）对大鼠进行预处理，当动物随后用完全弗氏佐剂中的经修饰大鼠雄性附属性腺进行攻击时，可显著降低对自身抗原的免疫反应。与对照动物（用大鼠肺生理盐水提取物或0.15M氯化钠预处理）相比，免疫前用低剂量抗原预处理的大鼠迟发型超敏反应明显受到抑制（P＜0.001）。受试雄性大鼠巨噬细胞迁移增强，而对照雄性大鼠的迁移受到抑制（P＜0.01）。在反应受到抑制的雄性大鼠中发现的迁移刺激表明存在迁移刺激因子，这被认为是抑制细胞活性的标志物。体液免疫也降低。用低剂量抗原对大鼠进行预处理可显著降低免疫反应，这可能是由于诱导了抑制细胞。
