CUHK(SZ)-Boyalife Joint Laboratory of Regenerative Medicine Engineering, School of Medicine, The Chinese University of Hong Kong, Shenzhen 518172, China.
Ciechanover Institute of Precision and Regenerative Medicine, School of Medicine, The Chinese University of Hong Kong, Shenzhen 518172, China.
ACS Sens. 2023 Sep 22;8(9):3520-3529. doi: 10.1021/acssensors.3c01093. Epub 2023 Sep 5.
A fully integrated device for salivary detection with a sample-in-answer-out fashion is critical for noninvasive point-of-care testing (POCT), especially for the screening of contagious disease infection. Microfluidic paper-based analytical devices (μPADs) have demonstrated their huge potential in POCT due to their low cost and easy adaptation with other components. This study developed a generic POCT platform by integrating a centrifugal microfluidic disc with μPADs to realize sample-to-answer salivary diagnostics. Specifically, a custom centrifugal microfluidic disc integrated with μPADs is fabricated, which demonstrated a high efficiency in saliva treatment. To demonstrate the capability of the integrated device for salivary analysis, the SARS-CoV-2 Nucleocapsid (N) protein, a reliable biomarker for SARS-CoV-2 acute infection, is used as the model analyte. By the chemical treatment of the μPAD surface, and by optimizing the protein immobilization conditions, the on-disc μPADs were able to detect the SARS-CoV-2 N protein down to 10 pg mL with a dynamic range of 10-1000 pg mL and an assay time of 8 min. The integrated device was successfully used for the quantification of the N protein of pseudovirus in saliva with high specificity and demonstrated a comparable performance to the commercial paper lateral flow assay test strips.
一种具有样本进结果出方式的唾液检测全集成装置对于非侵入性即时检测（POCT）至关重要，尤其适用于传染病感染的筛查。由于成本低且易于与其他组件结合，微流控纸基分析器件（μPADs）在 POCT 中显示出了巨大的潜力。本研究通过将离心微流控盘与 μPADs 集成，开发了一种通用的 POCT 平台，以实现样本至答案唾液诊断。具体来说，定制的离心微流控盘与 μPADs 集成，在唾液处理方面表现出高效率。为了展示集成设备进行唾液分析的能力，本研究使用 SARS-CoV-2 核衣壳（N）蛋白作为模型分析物，该蛋白是 SARS-CoV-2 急性感染的可靠生物标志物。通过 μPAD 表面的化学处理，并优化蛋白质固定化条件，盘上的 μPADs 能够检测到低至 10pg/mL 的 SARS-CoV-2 N 蛋白，检测范围为 10-1000pg/mL，检测时间为 8 分钟。该集成装置成功用于唾液中假病毒 N 蛋白的定量，具有高特异性，并表现出与商业纸质侧向流动检测试剂盒相当的性能。
