Department of Chemistry, Vel Tech Rangarajan Dr Sagunthala R & D Institute of Science and Technology, Avadi, Chennai, 600 062, Tamil Nadu, India.
Department of Chemistry, Vel Tech Rangarajan Dr Sagunthala R & D Institute of Science and Technology, Avadi, Chennai, 600 062, Tamil Nadu, India.
Talanta. 2021 Dec 1;235:122733. doi: 10.1016/j.talanta.2021.122733. Epub 2021 Jul 24.
The microfluidic paper-based analytical devices (μPADs) have grown-up swiftly over the decade due to its low cost, simple fabrication procedure, resource-limitedness, non-toxicity and their environmentally benign nature. The μPADs, also identified as point-of-care devices or health care devices have successfully applied in several fields such as diagnostics, biological, food safety, environmental, electrochemical and most importantly colorimetric/fluorimetric sensors, owing to the attractive passive motions of analyte without any external forces. In recent years, a large number of colorimetric and fluorimetric probes have been reported that can selectively recognize the analytes in μPADs. However, there is no organized review on its structure-activity relationship. In this review, we have focused to summarize the colorimetric and fluorimetric probes utilized in μPADs. This review discuss about the relationships between the structure and functions of various probes as signaling units of the efficient μPADs. The probes including nanomaterials, nanozymes, polymers and organic molecules, their structural activity with regard to sensing performances along with their limit of detection are also discussed. This review is expected to assist readers for better understanding of the sensing mechanisms of various chemo and bio-probes utilized in μPADs, as well as promote their advancement in the field. On the other hand, this review also helps the researchers for enhancement of μPADs and paves way for synergistic application of existing molecular probes as an effective diagnostic tool for the worldwide pandemic novel corona virus COVID-19.
微流控纸基分析器件（μPADs）由于其低成本、简单的制造工艺、资源有限、无毒和环境友好的特性，在过去十年中迅速发展。μPADs 也被称为即时检测设备或医疗保健设备，由于分析物在没有任何外力的情况下具有吸引人的被动运动，已成功应用于诊断、生物、食品安全、环境、电化学等多个领域，尤其是比色/荧光传感器。近年来，已经报道了大量可以选择性识别 μPADs 中分析物的比色和荧光探针。然而，目前还没有关于其结构-活性关系的系统综述。在这篇综述中，我们重点总结了用于 μPADs 的比色和荧光探针。本综述讨论了各种探针作为高效 μPADs 的信号单元的结构和功能之间的关系。讨论了探针包括纳米材料、纳米酶、聚合物和有机分子，它们的结构活性与其传感性能以及检测限之间的关系。本综述有望帮助读者更好地理解用于 μPADs 的各种化学和生物探针的传感机制，并促进它们在该领域的发展。另一方面，本综述还有助于研究人员增强 μPADs，并为现有分子探针的协同应用铺平道路，作为全球大流行新型冠状病毒 COVID-19 的有效诊断工具。
