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GTP酶RsgA介导的核糖体失活抑制T4噬菌体。

Ribosome inactivation by GTPase RsgA inhibits T4 phage.

作者信息

Fernández-García Laura, Tomás María, Wood Thomas K

机构信息

Department of Chemical Engineering, Pennsylvania State University, University Park, PA, United States.

Microbiology Translational and Multidisciplinary (MicroTM)-Research Institute Biomedical A Coruña (INIBIC) and Microbiology Department of Hospital A Coruña (CHUAC), University of A Coruña (UDC), A Coruña, Spain.

出版信息

Front Microbiol. 2023 Aug 21;14:1242163. doi: 10.3389/fmicb.2023.1242163. eCollection 2023.

DOI:10.3389/fmicb.2023.1242163
PMID:37670987
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10475562/
Abstract

INTRODUCTION

Bacteria must combat phages, and myriad bacterial anti-phage systems have been discovered that reduce host metabolism, for example, by depleting energetic compounds like ATP and NAD. Hence, these systems indirectly inhibit protein production. Surprisingly, direct reduction of ribosome activity has not been demonstrated to thwart phage.

METHODS

Here, by producing each of the 4,287 proteins and selecting for anti-phage activity that leads to enhanced growth, we investigated the role of host proteins in phage inhibition.

RESULTS AND DISCUSSION

We identified that GTPase RsgA inhibits lytic phage T4 by inactivating ribosomes.

摘要

引言

细菌必须对抗噬菌体，并且已经发现了无数种细菌抗噬菌体系统，这些系统会降低宿主代谢，例如通过消耗ATP和NAD等能量化合物。因此，这些系统会间接抑制蛋白质的产生。令人惊讶的是，尚未证明直接降低核糖体活性能够抵御噬菌体。

方法

在这里，我们通过产生4287种蛋白质中的每一种，并选择导致生长增强的抗噬菌体活性，来研究宿主蛋白在噬菌体抑制中的作用。

结果与讨论

我们发现GTP酶RsgA通过使核糖体失活来抑制裂解性噬菌体T4。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8c4/10475562/65ede2dfe341/fmicb-14-1242163-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8c4/10475562/ea7e52d22391/fmicb-14-1242163-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8c4/10475562/0f667489aca3/fmicb-14-1242163-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8c4/10475562/65ede2dfe341/fmicb-14-1242163-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8c4/10475562/ea7e52d22391/fmicb-14-1242163-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8c4/10475562/0f667489aca3/fmicb-14-1242163-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8c4/10475562/65ede2dfe341/fmicb-14-1242163-g003.jpg

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