Exercise Physiology Laboratory, Department of Physical Education, Yonsei University, Seoul 03722, Korea.
Department of Sports and Leisure Studies, School of Arts and Health, Myongji College, Seoul 03656, Korea.
Int J Environ Res Public Health. 2022 Sep 8;19(18):11292. doi: 10.3390/ijerph191811292.
Exercise can induce anti-inflammatory and antioxidant effects, for which regulation of sirtuins (SIRTs) may be a major consideration for exercise prescription. The purpose of this study was to investigate the effects of acute aerobic exercise, in particular its intensity, on systemic oxidative stress, inflammatory responses, and SIRT levels. Twenty healthy, untrained males were recruited and randomly assigned to moderate-intensity (MI, 65% VOmax, = 10) and high-intensity (HI, 85% VOmax, = 10) exercise. Blood samples were obtained pre-, immediately post-, and 1 h post-exercise for measurements of malonaldehyde (MDA), superoxide dis-mutase (SOD), interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, SIRT-1, SIRT-2, and SIRT-3. Overall, MDA, SOD, IL-6, SIRT-1, and SIRT-3 levels were significantly increased at post-exercise compared with pre-exercise regardless of exercise intensity ( < 0.05). The HI group had significantly higher MDA, SOD, and IL-6 levels than the MI group at post-exercise ( < 0.05), whereas no significant differences were observed in the IL-1β, TNF-α, and SIRT-2 levels ( > 0.05). Altogether, these findings suggest that exercise-induced oxidative stress and inflammatory responses may be dependent on exercise intensity. Moreover, activation of inflammatory cytokines and SIRT family members may be dependent on the intensity of the exercise.
运动可诱导抗炎和抗氧化作用，而 Sirtuins（SIRTs）的调节可能是运动处方的主要考虑因素。本研究旨在探讨急性有氧运动，特别是其强度，对全身氧化应激、炎症反应和 SIRT 水平的影响。招募了 20 名健康、未经训练的男性，并随机分为中等强度（MI，65%VOmax，n=10）和高强度（HI，85%VOmax，n=10）运动组。在运动前、运动后即刻和运动后 1 小时采集血样，用于测量丙二醛（MDA）、超氧化物歧化酶（SOD）、白细胞介素（IL）-1β、IL-6、肿瘤坏死因子（TNF）-α、SIRT-1、SIRT-2 和 SIRT-3。总的来说，无论运动强度如何（<0.05），运动后 MDA、SOD、IL-6、SIRT-1 和 SIRT-3 水平均明显高于运动前。与 MI 组相比，HI 组运动后 MDA、SOD 和 IL-6 水平显著升高（<0.05），而 IL-1β、TNF-α 和 SIRT-2 水平无显著差异（>0.05）。总之，这些发现表明，运动引起的氧化应激和炎症反应可能取决于运动强度。此外，炎性细胞因子和 SIRT 家族成员的激活可能依赖于运动的强度。
