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用于评估血液中氡和铅含量的生物传感器的制造。

Fabrication of biosensor for the assessment of radon and lead levels in the blood.

作者信息

Almayahi Basim A, Ali Amjad H

机构信息

Department of Physics, Faculty of Science, University of Kufa, Najaf, Iraq.

Department of Physics, Faculty of Science, Universiti Malaya, Kuala Lumpur, Malaysia.

出版信息

Heliyon. 2023 Aug 29;9(9):e19591. doi: 10.1016/j.heliyon.2023.e19591. eCollection 2023 Sep.

Abstract

This study aimed to develop and test a biosensor for detecting radioactive radon gas and lead ions in blood samples collected from donors in Iraq. The biosensor was made up of aptamer, acetic acid, malachite green, and TRIS-HAC, and results were measured using a fluorescence spectrophotometer. This study found that Rn in the blood varied between individuals, with higher levels in males and smokers, and the highest concentration found in a male patient with cancer. The biosensor used to detect Rn in the blood was effective, sensitive, and low-cost, and the levels detected were within the limits set by the WHO. The study also looked at pb, a toxic metal, and found that levels were within permissible limits. The biosensor was also effective in detecting pb. The correlations between the variables are generally weak to moderate, and there are some negative relationships between humidity and other variables. There are also some strong positive relationships between temperature (Tin) and temperature (Tout). The results suggest that these variables are not strongly correlated with each other, which is an important finding for understanding their potential effects on health outcomes. However, further validation and testing may be necessary before its widespread use in clinical settings. This study highlights the importance of monitoring these substances in the blood, especially for individuals with occupational exposure to radiation. The biosensor was found to be sensitive, cost-effective, fast to manufacture, and efficient compared to other detection devices. Therefore, the study recommends the use of this biosensor for measuring radon and lead ions in blood samples. The biosensor used in this study could be a useful tool for such monitoring.

摘要

本研究旨在开发并测试一种生物传感器,用于检测从伊拉克捐赠者采集的血液样本中的放射性氡气和铅离子。该生物传感器由适配体、乙酸、孔雀石绿和TRIS-HAC组成,并使用荧光分光光度计测量结果。本研究发现,血液中的氡含量因人而异,男性和吸烟者的含量较高,在一名男性癌症患者中发现的浓度最高。用于检测血液中氡的生物传感器有效、灵敏且成本低廉,检测到的水平在世界卫生组织设定的限值范围内。该研究还研究了有毒金属铅,发现其含量在允许范围内。该生物传感器在检测铅方面也很有效。变量之间的相关性一般较弱至中等,湿度与其他变量之间存在一些负相关关系。温度(Tin)和温度(Tout)之间也存在一些强正相关关系。结果表明,这些变量之间的相关性不强,这对于理解它们对健康结果的潜在影响是一个重要发现。然而,在临床环境中广泛使用之前,可能需要进一步的验证和测试。本研究强调了监测血液中这些物质的重要性,特别是对于有职业辐射暴露的个体。与其他检测设备相比,该生物传感器被发现灵敏、经济高效、制造快速且高效。因此,该研究建议使用这种生物传感器来测量血液样本中的氡和铅离子。本研究中使用的生物传感器可能是进行此类监测的有用工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0078/10480637/29135d8bf1c8/gr1.jpg

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