Fujian-Taiwan Science and Technology Cooperation Base of Biomedical Materials and Tissue Engineering, Engineering Research Center of Industrial Biocatalysis, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Chemistry and Materials Science, Fujian Normal University, Fuzhou 350007, China.
Fujian-Taiwan Science and Technology Cooperation Base of Biomedical Materials and Tissue Engineering, Engineering Research Center of Industrial Biocatalysis, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Chemistry and Materials Science, Fujian Normal University, Fuzhou 350007, China; State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100191, China.
J Control Release. 2023 Oct;362:577-590. doi: 10.1016/j.jconrel.2023.09.010. Epub 2023 Sep 10.
Bacterial infections and excessive inflammation can impede the healing of wounds. Hydrogels have emerged as a promising approach for dressing bacterial-infected injuries. However, some antibacterial hydrogels are complex, costly, and even require assistance with other instruments such as light, making them unsuitable for routine outdoor injuries. Here, we developed an in-situ generating hydrogel via hybridizing oxidized β-D-glucan with antimicrobial peptide CG through the Schiff base reaction. This hydrogel is easily accessible and actively contributes to the whole healing process of bacterial-infected wounds, demonstrating remarkable antibacterial activity and biological compatibility. The pH-sensitive reversible imine bond enables the hydrogel to self-heal and sustainably release the antibacterial peptide, thereby improving its bioavailability and reducing toxicity. Meanwhile, the immunoregulating β-D-glucan inhibits the release of inflammatory factors while promoting the release of anti-inflammatory factors. In methicillin-resistant Staphylococcus aureus (MRSA)-infected full-thickness skin wound models, the hybrid hydrogel showed superior antibacterial and anti-inflammatory activity, enhanced the M2 macrophage polarization, expedited wound closure, and regenerated epidermis tissue. These features make this hydrogel an appealing wound dressing for treating multi-drug-resistant bacteria-infected wounds.
细菌感染和过度炎症会阻碍伤口愈合。水凝胶作为一种有前途的方法，已经被用于治疗细菌感染的伤口。然而，一些抗菌水凝胶复杂、昂贵，甚至需要光等其他仪器的辅助，因此不适合常规的户外伤口。在这里，我们通过席夫碱反应将氧化的β-D-葡聚糖与抗菌肽 CG 杂交，开发了一种原位生成的水凝胶。这种水凝胶易于获得，并积极促进细菌感染伤口的整个愈合过程，表现出显著的抗菌活性和生物相容性。pH 敏感的可逆亚胺键使水凝胶能够自我修复和持续释放抗菌肽，从而提高其生物利用度并降低毒性。同时，免疫调节β-D-葡聚糖抑制炎症因子的释放，同时促进抗炎因子的释放。在耐甲氧西林金黄色葡萄球菌（MRSA）感染的全层皮肤伤口模型中，该杂交水凝胶表现出优异的抗菌和抗炎活性，增强了 M2 巨噬细胞的极化，加速了伤口闭合，并再生了表皮组织。这些特性使该水凝胶成为治疗多药耐药菌感染伤口的有吸引力的伤口敷料。
