Xiong Si, Li Ning, Shi Songshan, Zhao Yonglin, Chen Jie, Ruan Min, Xu Yongbin, Liu Ruimin, Wang Shunchun, Wang Huijun
The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Mate ria Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, PR China.
Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 725 Wan-Ping South Road, Shanghai 200032, China; Spine Institute, Shanghai University of Traditional Chinese Medicine, 725 Wan-Ping South Road, Shanghai 200032, China.
Int J Biol Macromol. 2024 Dec;283(Pt 3):137890. doi: 10.1016/j.ijbiomac.2024.137890. Epub 2024 Nov 19.
A novel polysaccharide SPS01-2 (87.5 kDa) was isolated from the roots of Scutellaria baicalensis Georgi. Monosaccharide composition revealed that SPS01-2 consists of rhamnose, arabinose, galactose, galacturonic acid, and glucuronic acid in ratio of 4.4: 67.1: 22.2: 6.3: 1.2. Further investigations using methylation, NMR, and mass spectrometry indicated that SPS01-2 is classified as a type II arabinogalactan (AG-II) with a minor presence of type I rhamnogalacturonan (RG-I). The core structure alternates between 1,2/1,2,4-α-L-Rhap and 1,4-α-D-GalpA, with branches including 1,3,6-β-D-Galp, 1,3-β-D-Galp, T-β-D-Galp, and T-α-L-Rhap. The RG-I regions are linked to 1,6-β-D-Galp, and 1,3,6-β-D-Galp units. Numerous arabinan branches, featuring multiple branching points, are attached to the O-3 position of galactose. Additionally, T-β-D-Galp, 1,6-β-D-Galp, and T-β-D-4-OMe-GlcpA are also linked to galactose in the backbone. Furthermore, SPS01-2 demonstrated potential immune-enhancing properties by dose-dependently increasing proliferation, phagocytosis, and the production of nitric oxide and cytokines (TNF-α, IL-6, and IL-1β) in RAW264.7 cells. It also enhanced the expression of CD80, CD86, and MHC-II at concentrations ranging from 5 to 200 μg/mL. Moreover, the immunostimulatory activity of SPS01-2 was significantly reduced when branch linkages were removed through partial acid hydrolysis. Our findings indicate that SPS01-2 could serve as a natural immunostimulant in the food and pharmaceutical sectors.
从黄芩根部分离出一种新型多糖SPS01-2（87.5 kDa）。单糖组成显示，SPS01-2由鼠李糖、阿拉伯糖、半乳糖、半乳糖醛酸和葡萄糖醛酸组成，其比例为4.4:67.1:22.2:6.3:1.2。使用甲基化、核磁共振和质谱进行的进一步研究表明，SPS01-2被归类为II型阿拉伯半乳聚糖（AG-II），少量存在I型鼠李糖半乳糖醛酸聚糖（RG-I）。核心结构在1,2/1,2,4-α-L-鼠李糖和1,4-α-D-半乳糖醛酸之间交替，分支包括1,3,6-β-D-半乳糖、1,3-β-D-半乳糖、T-β-D-半乳糖和T-α-L-鼠李糖。RG-I区域与1,6-β-D-半乳糖和1,3,6-β-D-半乳糖单元相连。许多具有多个分支点的阿拉伯聚糖分支连接到半乳糖的O-3位置。此外，T-β-D-半乳糖、1,6-β-D-半乳糖和T-β-D-4-O-甲基葡萄糖醛酸也连接到主链中的半乳糖上。此外，SPS01-2通过剂量依赖性地增加RAW264.7细胞中的增殖、吞噬作用以及一氧化氮和细胞因子（TNF-α、IL-6和IL-1β）的产生，表现出潜在的免疫增强特性。它还在5至200μg/mL的浓度范围内增强了CD80、CD86和MHC-II的表达。此外，通过部分酸水解去除分支连接后，SPS01-2的免疫刺激活性显著降低。我们的研究结果表明，SPS01-2可作为食品和制药行业的天然免疫刺激剂。
