Brzozowa-Zasada Marlena, Piecuch Adam, Michalski Marek, Stęplewska Katarzyna, Matysiak Natalia, Kucharzewski Marek
Department of Histology and Cell Pathology in Zabrze, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, 40-055 Katowice, Poland.
Department of Pathology, Institute of Medical Sciences, University of Opole, 45-052 Opole, Poland.
J Clin Med. 2023 Aug 23;12(17):5477. doi: 10.3390/jcm12175477.
(1) Background: Colorectal cancer (CRC) is the third most common cancer in terms of incidence and mortality. Approximately 90% of all colorectal cancer cases are adenocarcinomas, originating from epithelial cells of the colorectal mucosa. Upregulated gene 4 (URG4) is an oncogene involved in cancer development. The aim of the study was to assess the immunohistochemical expression of URG4 protein expression in Polish patients with colon adenocarcinoma who were not treated with any therapy before radical surgery. (2) Methods: The study used colon tissue samples taken from people with a confirmed diagnosis of colorectal adenocarcinoma after a thorough histopathological examination. The associations between the immunohistochemical expression of URG4 and clinical parameters were analyzed by the Chi2 test or Chi2Yatesa test. The study conducted an analysis of the correlation between the expression of URG4 and the five-year survival rate of patients through the application of the Kaplan-Meier analysis and the log-rank statistical test. The intracellular localization of URG4 was identified through the utilization of transmission electron microscopy (TEM) methodology. (3) Results: In univariate Cox regression analyses, immuno-histochemical expression of URG4, grade of histological differentiation, depth of invasion, angioinvasion, PCNA expression, stage of disease and lymph node involvement were found to be significant prognostic factors. Within our patient cohort, it was observed that the degree of tumour differentiation and URG4 expression were found to be distinct prognostic factors in regard to the 5-year survival rates of those with colon adenocarcinoma. (4) Conclusions: High immunohistochemical expression of URG4 correlates with poor prognosis in patients with colon adenocarcinoma.
(1) 背景：结直肠癌（CRC）在发病率和死亡率方面是第三大常见癌症。所有结直肠癌病例中约90%为腺癌，起源于结直肠黏膜的上皮细胞。上调基因4（URG4）是一种参与癌症发展的癌基因。本研究的目的是评估未经任何治疗的波兰结肠腺癌患者在根治性手术前URG4蛋白表达的免疫组化情况。(2) 方法：本研究使用了经彻底组织病理学检查确诊为结直肠腺癌患者的结肠组织样本。通过卡方检验或卡方耶茨检验分析URG4免疫组化表达与临床参数之间的关联。本研究通过应用Kaplan-Meier分析和对数秩统计检验对URG4表达与患者五年生存率之间的相关性进行了分析。通过透射电子显微镜（TEM）方法确定URG4的细胞内定位。(3) 结果：在单变量Cox回归分析中，发现URG4的免疫组化表达、组织学分化程度、浸润深度、血管浸润、PCNA表达、疾病分期和淋巴结受累是显著的预后因素。在我们的患者队列中，观察到肿瘤分化程度和URG4表达是结肠腺癌患者五年生存率的不同预后因素。(4) 结论：URG4的高免疫组化表达与结肠腺癌患者的不良预后相关。
