Lian Huida, Qin Cheng, Shen Jie, Ahanger Mohammad Abass
Department of Life Sciences, University of Changzhi, Changzhi 046000, China.
College of Life Science, Northwest A&F University, Yangling 712100, China.
Plants (Basel). 2023 Aug 28;12(17):3082. doi: 10.3390/plants12173082.
The influence of drought induced by polyethylene glycol (PEG) and the alleviatory effect of nitric oxide (50 µM) and sulphur (S, 1 mM KSO) were studied in . Drought stress reduced plant height, dry weight, total chlorophylls, carotenoids and the content of nitrogen, phosphorous, potassium and sulphur. The foliar applications of NO and sulphur each individually alleviated the decline, with a greater alleviation observed in seedlings treated with both NO and sulphur. The reduction in intermediates of chlorophyll synthesis pathways and photosynthesis were alleviated by NO and sulphur. Oxidative stress was evident through the increased hydrogen peroxide, superoxide and activity of lipoxygenase and protease which were significantly assuaged by NO, sulphur and NO + sulphur treatments. A reduction in the activity of nitrate reductase, glutamine synthetase and glutamate synthase was mitigated due to the application of NO and the supplementation of sulphur. The endogenous concentration of NO and hydrogen sulphide (HS) was increased due to PEG; however, the PEG-induced increase in NO and HS was lowered due to NO and sulphur. Furthermore, NO and sulphur treatments to PEG-stressed seedlings further enhanced the functioning of the antioxidant system, osmolytes and secondary metabolite accumulation. Activities of γ-glutamyl kinase and phenylalanine ammonia lyase were up-regulated due to NO and S treatments. The treatment of NO and S regulated the expression of the , , , and genes significantly under normal and drought stress. The present study advocates for the beneficial use of NO and sulphur in the mitigation of drought-induced alterations in the metabolism of .
研究了聚乙二醇（PEG）诱导的干旱影响以及一氧化氮（50 µM）和硫（S，1 mM KSO）的缓解作用。干旱胁迫降低了株高、干重、总叶绿素、类胡萝卜素以及氮、磷、钾和硫的含量。叶面单独施用NO和硫均可缓解下降情况，同时施用NO和硫的幼苗缓解效果更明显。NO和硫缓解了叶绿素合成途径和光合作用中间产物的减少。通过过氧化氢、超氧化物以及脂氧合酶和蛋白酶活性的增加可明显看出氧化应激，而NO、硫以及NO +硫处理可显著缓解这种应激。由于施用NO和补充硫，硝酸还原酶、谷氨酰胺合成酶和谷氨酸合酶的活性降低得到缓解。PEG导致NO和硫化氢（HS）的内源浓度增加；然而，由于NO和硫的作用，PEG诱导的NO和HS增加有所降低。此外，对PEG胁迫的幼苗进行NO和硫处理进一步增强了抗氧化系统的功能、渗透调节物质和次生代谢产物的积累。由于NO和S处理，γ-谷氨酰激酶和苯丙氨酸解氨酶的活性上调。在正常和干旱胁迫下，NO和S处理显著调节了 、 、 、 和 基因的表达。本研究主张有益地利用NO和硫来缓解干旱诱导的 代谢变化。
