Li Yongliang, Li Guanwen, Deng Xuliang
Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology, Beijing, People's Republic of China.
National Engineering Laboratory for Digital and Material Technology of Stomatology, NMPA Key Laboratory for Dental Materials, Beijing Laboratory of Biomedical Materials, Peking University School and Hospital of Stomatology, Beijing, People's Republic of China.
J Oral Microbiol. 2023 Sep 7;15(1):2253675. doi: 10.1080/20002297.2023.2253675. eCollection 2023.
Effective control of gene expression is crucial for understanding gene function in both eukaryotic and prokaryotic cells. While several inducible gene expression systems have been reported in , a conditional pathogen that causes dental caries, the significant non-inducible basal expression in these systems seriously limits their utility, especially when studying lethal gene functions and molecular mechanisms. We introduce a tightly controlled xylose-inducible gene expression system, TC-Xyl, for . Western blot results and fluorescence microscopy analysis indicate that TC-Xyl exhibits an extremely low non-inducible basal expression level and a sufficiently high expression level post-induction. Further, by constructing a mutation in which the only source FtsZ is under the control of TC-Xyl, we preliminarily explored the function of the gene. We found that FtsZ depletion is lethal to , resulting in abnormal round cell shape and mini cell formation, suggesting FtsZ's role in maintaining cell shape stability.
有效控制基因表达对于理解真核细胞和原核细胞中的基因功能至关重要。虽然在一种导致龋齿的条件性病原体中已经报道了几种可诱导基因表达系统，但这些系统中显著的非诱导性基础表达严重限制了它们的实用性，特别是在研究致死基因功能和分子机制时。我们为[具体病原体名称未给出]引入了一种严格控制的木糖诱导基因表达系统，即TC-Xyl。蛋白质印迹结果和荧光显微镜分析表明，TC-Xyl表现出极低的非诱导性基础表达水平和诱导后足够高的表达水平。此外，通过构建一种突变体，其中唯一的FtsZ来源受TC-Xyl控制，我们初步探索了[具体基因名称未给出]基因的功能。我们发现FtsZ缺失对[具体病原体名称未给出]是致命的，导致细胞形状异常呈圆形并形成微小细胞，这表明FtsZ在维持细胞形状稳定性中起作用。
