Tu Ran, Mao Yufeng, Liu Ye, Cheng Haijiao, Yuan Wei, Yu Sili, Pan Wenjia, An Jingjing, Wang Meng
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
National Technology Innovation Center of Synthetic Biology, Tianjin 300308, China.
Sheng Wu Gong Cheng Xue Bao. 2022 Nov 25;38(11):4162-4179. doi: 10.13345/j.cjb.220582.
One of the revolutionary features of synthetic biology is that the standardization and modularization of biological experimental objects, methods, technologies and processes can be combined with various software and hardware to forge into an automated high-throughput synthetic biology biofoundry. Disrupting the conventional labor-intensive research paradigm, biofoundry represents a novel research paradigm with highly enhanced technical iteration capabilities, and remarkably promotes the development and industrial applications of synthetic biology. On the occasion of the 10th anniversary of the founding of Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, this review summarized a series of important achievements in the field of automated high-throughput editing and screening of industrial strains. These achievements range from automated editing technologies such as gene cloning, genome editing, and editing sequence design, to high-throughput screening technologies such as fluorescence activated cell sorting, fluorescence activated droplet sorting, and genome-scale gene perturb sequencing. Moreover, we prospected future development of this field, hoping to provide overall support for intelligent, automated and full chain integrated creation of excellent industrial strains with intellectual property rights.
合成生物学的革命性特征之一在于，生物实验对象、方法、技术及流程的标准化与模块化能够与各种软硬件相结合，打造出自动化的高通量合成生物学造药厂。造药厂打破了传统的劳动密集型研究模式，代表着一种技术迭代能力大幅增强的新型研究模式，并显著推动了合成生物学的发展及工业应用。值中国科学院天津工业生物技术研究所成立十周年之际，本综述总结了工业菌株自动化高通量编辑与筛选领域的一系列重要成果。这些成果涵盖从基因克隆、基因组编辑及编辑序列设计等自动化编辑技术，到荧光激活细胞分选、荧光激活液滴分选及基因组规模基因扰动测序等高通量筛选技术。此外，我们展望了该领域的未来发展，希望为具有知识产权的优良工业菌株的智能化、自动化及全链条集成创制提供全面支撑。
