State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, 100871, China.
State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, CAS Center for Excellence in Molecular Plant Sciences, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
Nat Commun. 2023 Sep 13;14(1):5673. doi: 10.1038/s41467-023-41416-1.
Abnormal high temperature (HT) caused by global warming threatens plant survival and food security, but the effects of HT on plant organ identity are elusive. Here, we show that Class II TEOSINTE BRANCHED 1/CYCLOIDEA/ PCF (TCP) transcription factors redundantly protect ovule identity under HT. The duodecuple tcp2/3/4/5/10/13/17/24/1/12/18/16 (tcpDUO) mutant displays HT-induced ovule conversion into carpelloid structures. Expression of TCP4 in tcpDUO complements the ovule identity conversion. TCP4 interacts with AGAMOUS (AG), SEPALLATA3 (SEP3), and the homeodomain transcription factor BELL1 (BEL1) to strengthen the association of BEL1 with AG-SEP3. The tcpDUO mutant synergistically interacts with bel1 and the ovule identity gene seedstick (STK) mutant stk in tcpDUO bel1 and tcpDUO stk. Our findings reveal the critical roles of Class II TCPs in maintaining ovule identity under HT and shed light on the molecular mechanisms by which ovule identity is determined by the integration of internal factors and environmental temperature.
异常高温（HT）由全球变暖引起，威胁着植物的生存和粮食安全，但 HT 对植物器官身份的影响还不清楚。在这里，我们表明，第二类 TEOSINTE BRANCHED 1/CYCLOIDEA/PCF（TCP）转录因子在 HT 下冗余地保护胚珠身份。十二倍的 tcp2/3/4/5/10/13/17/24/1/12/18/16（tcpDUO）突变体显示出 HT 诱导的胚珠转化为心皮状结构。在 tcpDUO 中表达 TCP4 可补充胚珠身份转换。TCP4 与 AGAMOUS（AG）、SEPALLATA3（SEP3）和同源域转录因子 BELL1（BEL1）相互作用，增强 BEL1 与 AG-SEP3 的结合。tcpDUO 突变体与 bel1 和胚珠身份基因 seedstick（STK）突变体 stk 在 tcpDUO bel1 和 tcpDUO stk 中协同相互作用。我们的发现揭示了第二类 TCP 在维持 HT 下胚珠身份方面的关键作用，并阐明了胚珠身份是如何通过内部因素和环境温度的整合来决定的分子机制。
