Computational Optics Group, University of Tsukuba, Tsukuba, Ibaraki, 305-8573, Japan.
Department of Physics, Faculty of Science, Damietta University, New Damietta City, Damietta, 34517, Egypt.
Sci Rep. 2023 Sep 16;13(1):15377. doi: 10.1038/s41598-023-41846-3.
This study aims at demonstrating label-free drug-response-patterns assessment of different tumor spheroids and drug types by dynamic optical coherence tomography (D-OCT). The study involved human breast cancer (MCF-7) and colon cancer (HT-29) spheroids. The MCF-7 and HT-29 spheroids were treated with paclitaxel (Taxol; PTX) and the active metabolite of irinotecan SN-38, respectively. The drugs were applied with 0 (control), 0.1, 1, and 10 μM concentrations and the treatment durations were 1, 3, and 6 days. A swept-source OCT microscope equipped with a repeated raster scanning protocol was used to scan the spheroids. Logarithmic intensity variance (LIV) and late OCT correlation decay speed (OCDS[Formula: see text]) algorithms were used to visualize the tumor spheroid dynamics. LIV and OCDS[Formula: see text] images visualized different response patterns of the two types of spheroids. In addition, spheroid morphology, LIV, and OCDS[Formula: see text] quantification showed different time-courses among the spheroid and drug types. These results may indicate different action mechanisms of the drugs. The results showed the feasibility of D-OCT for the evaluation of drug response patterns of different cell spheroids and drug types and suggest that D-OCT can perform anti-cancer drug testing.
本研究旨在通过动态光学相干断层扫描（D-OCT）来证明对不同肿瘤球体和药物类型进行无标记药物反应模式评估的可能性。该研究涉及人乳腺癌（MCF-7）和结肠癌（HT-29）球体。MCF-7 和 HT-29 球体分别用紫杉醇（PTX）和伊立替康的活性代谢物 SN-38 处理。药物以 0（对照）、0.1、1 和 10 μM 浓度应用，处理时间分别为 1、3 和 6 天。使用配备重复光栅扫描协议的扫频源 OCT 显微镜扫描球体。对数强度方差（LIV）和晚期 OCT 相关衰减速度（OCDS[公式：见正文]）算法用于可视化肿瘤球体动力学。LIV 和 OCDS[公式：见正文]图像可视化了两种类型球体的不同反应模式。此外，球体形态、LIV 和 OCDS[公式：见正文]定量显示了不同球体和药物类型之间的不同时间过程。这些结果可能表明药物的作用机制不同。结果表明 D-OCT 可用于评估不同细胞球体和药物类型的药物反应模式，并表明 D-OCT 可进行抗癌药物测试。
