Huang Zhijiong, Yao Hongyu, Yang Zhijun
Department of Gynecological Oncology, Guangxi Medical University Cancer Hospital, Nanning, Guangxi 530021, P.R. China.
Key Laboratory of Early Prevention and Treatment for Regional High Frequency Tumor (Guangxi Medical University), Ministry of Education, Nanning, Guangxi 530021, P.R. China.
Oncol Lett. 2023 Aug 30;26(4):448. doi: 10.3892/ol.2023.14035. eCollection 2023 Oct.
Transmembrane 4 L6 family member 1 (TM4SF1) and discoidin domain receptor 1 (DDR1) are expressed in numerous types of cancer, but their expression in epithelial ovarian cancer and the association between their expression and patient prognosis are unclear. The present study aimed to explore the expression of TM4SF1 and DDR1 and their relationship with prognosis in epithelial ovarian cancer. Firstly, the Oncomine and Gene Expression Profiling Interactive Analysis (GEPIA) platforms were used to compare the expression levels of TM4SF1 and DDR1 in ovarian cancer and normal ovarian tissue, and Kaplan-Meier plotter was used to analyze the association between gene expression and patient prognosis. The proteins interacting with TM4SF1 and DDR1 were analyzed using Search Tool for the Retrieval of Interacting Genes/Proteins (STRING), and enrichment analysis of Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways was conducted for the interacting proteins. Furthermore, immunohistochemical staining was performed to detect the expression of TM4SF1 and DDR1 protein in epithelial ovarian cancer tissue and to analyze the association between expression and prognosis. The Oncomine and GEPIA analyses showed that the expression levels of TM4SF1 and DDR1 were significantly higher in epithelial ovarian cancer than in normal ovarian tissue, and the analysis of clinical samples revealed that TM4SF1 and DDR1 were coexpressed in some cases. STRING analysis indicated that the TM4SF1 and DDR1 proteins interact with each other. The overall survival and progression-free survival of patients whose epithelial ovarian cancer coexpressed TM4SF1 and DDR1 were significantly shorter than those of patients lacking TM4SF1 and DDR1 coexpression. Multivariate analysis indicated that TM4SF1 and DDR1 protein coexpression was an independent prognostic factor. In summary, TM4SF1 and DDR1 proteins were coexpressed in some epithelial ovarian cancer tissues and appear to be adverse prognostic factors for epithelial ovarian cancer. In addition, TM4SF1 and DDR1 may have an interactive or mutual regulatory mechanism.
跨膜4 L6家族成员1（TM4SF1）和盘状结构域受体1（DDR1）在多种癌症类型中均有表达，但其在上皮性卵巢癌中的表达情况以及它们的表达与患者预后之间的关联尚不清楚。本研究旨在探讨TM4SF1和DDR1在上皮性卵巢癌中的表达及其与预后的关系。首先，利用Oncomine和基因表达谱交互分析（GEPIA）平台比较TM4SF1和DDR1在卵巢癌组织与正常卵巢组织中的表达水平，并使用Kaplan-Meier绘图工具分析基因表达与患者预后之间的关联。使用检索相互作用基因/蛋白质的搜索工具（STRING）分析与TM4SF1和DDR1相互作用的蛋白质，并对相互作用的蛋白质进行基因本体论和京都基因与基因组百科全书通路的富集分析。此外，进行免疫组织化学染色以检测TM4SF1和DDR1蛋白在上皮性卵巢癌组织中的表达，并分析表达与预后之间的关联。Oncomine和GEPIA分析显示，TM4SF1和DDR1在上皮性卵巢癌中的表达水平显著高于正常卵巢组织，临床样本分析显示，TM4SF1和DDR1在某些病例中共同表达。STRING分析表明，TM4SF1和DDR1蛋白相互作用。上皮性卵巢癌共同表达TM4SF1和DDR1的患者的总生存期和无进展生存期明显短于未共同表达TM4SF1和DDR1的患者。多因素分析表明，TM4SF1和DDR1蛋白共同表达是一个独立的预后因素。总之，TM4SF1和DDR1蛋白在一些上皮性卵巢癌组织中共同表达，似乎是上皮性卵巢癌的不良预后因素。此外，TM4SF1和DDR1可能具有相互作用或相互调节的机制。
